Separation of total lipids on human lipoproteins using surfactant-coated multiwalled carbon nanotubes as pseudostationary phase in capillary electrophoresis

Mei Yu Su, Yen Yi Chen, Jian Ying Yang, You Sian Lin, Yang Wei Lin, Mine Yine Liu

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

Surfactant-coated multiwalled carbon nanotubes (MWNTs) were used as pseudostationary phase (PSP) in CE to investigate the total lipids of high-density lipoproteins and low-density lipoproteins. To optimize the CE conditions, several experimental factors including carbon nanotube concentration, bile salt concentration, sodium phosphate (PB) concentration, organic modifier concentration and buffer pH value have been examined. In addition, the CE capillary temperature and applied voltage have also been examined. The optimal separation buffer selected was a mixture of 3.2 mg/L MWNT, 50 mM bile salt, 10 mM PB, 20% 1-propanol, pH 9.5. The optimal capillary temperature and applied voltage selected were 50°C and 20 kV, respectively. Phosphatidyl choline (PC) has been used as a model analyte and investigated by the optimal CE method. The linear range for PC was 0.1-3 mg/mL with a correlation coefficient of 0.9934, and the concentration LOD was 0.055 mg/mL. The optimal CE method has been used to characterize the total lipids of high-density lipoprotein and low-density lipoprotein. At absorbance 200 nm, one major peak and two or three minor peaks showed for the total lipids of lipoproteins within 13 minutes. Resolutions of the total lipids were enhanced using surfactant-coated MWNTs as PSPs in the CE separation buffer. However, resolutions of the total lipids were not enhanced using surfactant-coated single-walled carbon nanotubes as PSPs in the CE separation buffer.

原文English
頁(從 - 到)978-985
頁數8
期刊Electrophoresis
35
發行號7
DOIs
出版狀態Published - 2014 四月

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

指紋 深入研究「Separation of total lipids on human lipoproteins using surfactant-coated multiwalled carbon nanotubes as pseudostationary phase in capillary electrophoresis」主題。共同形成了獨特的指紋。

引用此