Separation and determination of in vitro oxidized phospholipids by capillary zone electrophoresis

Yu Ling Ho, Jing Huei Chiu, Chung Yu Wu, Mine Yine Liu

研究成果: Article

13 引文 斯高帕斯(Scopus)

摘要

A simple capillary zone electrophoresis (CZE) method was used to determine in vitro oxidized phosphatidyl choline (ox-PC). To optimize the capillary electrophoresis (CE) conditions, organic buffer additives, buffer ionic strength, buffer pH, and applied voltage were examined. The optimal CE separation buffer chosen was an aqueous-organic solvent system containing 10% sodium phosphate buffer (5 mM, pH 7.40), 80% methanol, and 10% acetonitrile. One major peak with a small shoulder was found for phosphatidyl choline (PC), whereas one major peak and a complex region containing several lower-mobility peaks were found for ox-PC. The lower-mobility species of ox-PC has high levels of conjugated dienes characterized by strong absorbance at 234 nm. The electropherograms of PC and ox-PC were significantly different and highly reproducible. The intensities of lower-mobility species decreased significantly when the antioxidant vitamin C concentration was increased from 6 to 600 μM. This study provides a simple CZE method to differentiate in vitro oxidized from nonoxidized PC molecular species.

原文English
頁(從 - 到)210-218
頁數9
期刊Analytical Biochemistry
367
發行號2
DOIs
出版狀態Published - 2007 八月 15

    指紋

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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