Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409

Chi Wen Lin, Dang Thuan Tran, Chi Yung Lai, Yet Pole I, Chih Hung Wu

研究成果: Article

19 引文 (Scopus)

摘要

The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.

原文English
頁(從 - 到)1922-1929
頁數8
期刊Biomass and Bioenergy
34
發行號12
DOIs
出版狀態Published - 2010 十二月 1

指紋

Klebsiella oxytoca
Pennisetum
ethanol production
ethanol
Ethanol
yeast extract
Yeast
Fermentation
fermentation
yeast
lignocellulose
response surface methodology
powders
temperature
regression analysis
Regression analysis
microorganisms
Productivity
liquids
Powders

All Science Journal Classification (ASJC) codes

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

引用此文

Lin, Chi Wen ; Tran, Dang Thuan ; Lai, Chi Yung ; I, Yet Pole ; Wu, Chih Hung. / Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409. 於: Biomass and Bioenergy. 2010 ; 卷 34, 編號 12. 頁 1922-1929.
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Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409. / Lin, Chi Wen; Tran, Dang Thuan; Lai, Chi Yung; I, Yet Pole; Wu, Chih Hung.

於: Biomass and Bioenergy, 卷 34, 編號 12, 01.12.2010, p. 1922-1929.

研究成果: Article

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AU - Wu, Chih Hung

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AB - The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.

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