Quantification of Captopril in Urine Through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Using 4-Mercaptobenzoic Acid-Capped Gold Nanoparticles as an Internal Standard

Wen Tsen Chen, Cheng Kang Chiang, Yang Wei Lin, Huan Tsung Chang

研究成果: Article

38 引文 斯高帕斯(Scopus)


We have developed a new internal standard method for the determination of the concentration of captopril (CAP) through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using gold nanoparticles (Au NPs). This approach provided linearity for CAP over the concentration range 2.525 M (R2 = 0.987), with a limit of detection (signal-to-noise ratio = 3) of 1.0 M. The spot-to-spot variations in the concentration of CAP through SALDI-MS analyses performed in the absence and presence of the internal standard were 26% and 9%, respectively (15 measurements). This approach provides simplicity, accuracy, precision, and great reproducibility to the determination of the levels of CAP in human urine samples.

頁(從 - 到)864-867
期刊Journal of the American Society for Mass Spectrometry
出版狀態Published - 2010 五月 1


All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Spectroscopy