In this research, a multi-function electro-cardiogram measurement system is designed and constructed. The system applies the structure of virtual instrument built by software part, Labviewinverted commas hardware part, DAQ card and some external circuits is applied in the development process. This research integrates different fields that include physiological signals, circuits, virtual instrument, and physiology. The functions of system include waveform display, auto signal processing, data saving, histogram display, heartbeat display, irregular heart rhythms warning and remote measurement. Practical measurements have been carried out to demonstrate the capabilities of this measurement technique.