Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing

Pin Quan Hou, Yung I. Lee, Kuei Ting Hsu, Yu Ting Lin, Wei Zhi Wu, John Yee Lin, Trinh Ngoc Nam, Shih Feng Fu

研究成果: Article

8 引文 斯高帕斯(Scopus)

摘要

DNA methylation is essential for normal developmental processes and genome stability. DNA methyltransferases are key enzymes catalyzing DNA methylation. Chromomethylase (CMT) genes are specific to the plant kingdom and encode chromodomain-containing methyltransferases. However, the function of CMT genes in plants remains elusive. In this study, we isolated and characterized a CMT gene from Nicotiana benthamiana, designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with other plant CMT3s showed conservation of bromo-adjacent-homology and methyltransferase catalytic domains. We investigated the expression patterns of NbCMT3 and its function in developmental programs. NbCMT3 was expressed predominately in proliferating tissues such as apical shoots and young leaves. NbCMT3 protein showed a nuclear location, which could be related to its putative cellular functions. Knocking down NbCMT3 expression by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis. The formation of palisade cells was defective in NbCMT3-silenced plants as compared with controls. NbCMT3 has a role in developmental programs.

原文English
頁(從 - 到)119-132
頁數14
期刊Physiologia Plantarum
150
發行號1
DOIs
出版狀態Published - 2014 一月 1

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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