Fiber-optic multiphoton flow cytometry in whole blood and in vivo

Yu Chung Chang, Jing Yong Ye, Thommey P. Thomas, Zhengyi Cao, Alina Kotlyar, Eric R. Tkaczyk, James R. Baker

研究成果: Article

29 引文 斯高帕斯(Scopus)

摘要

Circulating tumor cells in the bloodstream are sensitive indicators for metastasis and disease prognosis. Circulating cells have usually been monitored via extraction from blood, and more recently in vivo using freespace optics; however, long-term intravital monitoring of rare circulating cells remains a major challenge. We demonstrate the application of a two-photon-fluorescence optical fiber probe for the detection of cells in whole blood and in vivo. A double-clad fiber was used to enhance the detection sensitivity. Two-channel detection was employed to enable simultaneous measurement of multiple fluorescent markers. Because the fiber probe circumvents scattering and absorption from whole blood, the detected signal strength from fluorescent cells was found to be similar in phosphate-buffered saline (PBS) and in whole blood. The detection efficiency of cells labeled with the membrane-binding dye 1,1(-dioctadecyl-3,3,3',3'- tetramethylindoldicarbocyanine, 4- chlorobenzenesulfonate (DiD) was demonstrated to be the same in PBS and in whole blood. A high detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was also demonstrated. To characterize in vivo detection, DiDlabeled untransfected and GFP-transfected cells were injected into live mice, and the cell circulation dynamics was monitored in real time. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed ex vivo in whole blood.

原文English
文章編號047004
期刊Journal of Biomedical Optics
15
發行號4
DOIs
出版狀態Published - 2010 七月 1

    指紋

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

引用此

Chang, Y. C., Ye, J. Y., Thomas, T. P., Cao, Z., Kotlyar, A., Tkaczyk, E. R., & Baker, J. R. (2010). Fiber-optic multiphoton flow cytometry in whole blood and in vivo. Journal of Biomedical Optics, 15(4), [047004]. https://doi.org/10.1117/1.3463481