Demonstration of expression of a neuropeptide-encoding gene in crustacean hemocytes

Su Hua Wu, Yan Jhou Chen, Shao Yen Huang, Wei Shiun Tsai, Hsin Ju Wu, Tsan Ting Hsu, Chi Ying Lee

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

Crustacean hyperglycemic hormone (CHH) was originally identified in a neuroendocrine system-the X-organ/sinus gland complex. In this study, a cDNA (Prc-CHH) encoding CHH precursor was cloned from the hemocyte of the crayfish Procambarus clarkii. Analysis of tissues by a CHH-specific enzyme-linked immunosorbent assay (ELISA) confirmed the presence of CHH in hemocytes, the levels of which were much lower than those in the sinus gland, but 2 to 10 times higher than those in the thoracic and cerebral ganglia. Total hemocytes were separated by density gradient centrifugation into layers of hyaline cell (HC), semi-granular cell (SGC), and granular cell (GC). Analysis of extracts of each layer using ELISA revealed that CHH is present in GCs (202.8. ±. 86.7. fmol/mg protein) and SGCs (497.8. ±. 49.4. fmol/mg protein), but not in HCs. Finally, CHH stimulated the membrane-bound guanylyl cyclase (GC) activity of hemocytes in a dose-dependent manner. These data for the first time confirm that a crustacean neuropeptide-encoding gene is expressed in cells essential for immunity and its expression in hemocytes is cell type-specific. Effect of CHH on the membrane-bound GC activity of hemocyte suggests that hemocyte is a target site of CHH. Possible functions of the hemocyte-derived CHH are discussed.

原文English
頁(從 - 到)463-468
頁數6
期刊Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
161
發行號4
DOIs
出版狀態Published - 2012 四月

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

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