Cell flow analysis with a two-photon fluorescence fiber probe

Yu Chung Chang, Jing Yong Ye, Thommey P. Thomas, James R. Baker, Theodore B. Norris

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

We report the use of a sensitive double-clad fiber (DCF) probe for in situ cell flow velocity measurements and cell analysis by means of two-photon excited fluorescence correlation spectroscopy (FCS). We have demonstrated the feasibility to use this fiber probe for in vivo two-photon flow cytometry previously. However, because of the viscosity of blood and the non-uniform flow nature in vivo, it is problematic to use the detected cell numbers to estimate the sampled blood volume. To precisely calibrate the sampled blood volume, it is necessary to conduct real time flow velocity measurement. We propose to use FCS technique to measure the flow velocity. The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. Our two-photon fluorescence fiber probe has the ability to monitor multiple fluorescent biomarkers simultaneously. We demonstrate that we can distinguish differently labeled cells by their distinct features on the correlation curves. The ability to conduct in situ cell flow analysis using the fiber probe may be useful in disease diagnosis or further comprehension of the circulation system.

原文English
主出版物標題Optics in Health Care and Biomedical Optics IV
DOIs
出版狀態Published - 2010 十二月 29
事件Optics in Health Care and Biomedical Optics IV - Beijing, China
持續時間: 2010 十月 182010 十月 20

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
7845
ISSN(列印)1605-7422

Other

OtherOptics in Health Care and Biomedical Optics IV
國家China
城市Beijing
期間10-10-1810-10-20

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • 引用此

    Chang, Y. C., Ye, J. Y., Thomas, T. P., Baker, J. R., & Norris, T. B. (2010). Cell flow analysis with a two-photon fluorescence fiber probe. 於 Optics in Health Care and Biomedical Optics IV [78450U] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; 卷 7845). https://doi.org/10.1117/12.870295