Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves

Kuan Chou Chen, Chiu Lan Hsieh, Kuan Dar Huang, Yaw Bee Ker, Charng Cherng Chyau, Robert Y. Peng

研究成果: Article同行評審

20 引文 斯高帕斯(Scopus)

摘要

Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.

原文English
頁(從 - 到)6114-6122
頁數9
期刊Journal of Agricultural and Food Chemistry
57
發行號14
DOIs
出版狀態Published - 2009 七月 22

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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