It is important to study the osteoblast and chondrocyte differentiations of human mesenchymal stem cells (hMSCs) due to a significant demand for prerequisite regenerative medicine. Many physical stimulators would also promote cell differentiation. This study presents a time-efficient cell differentiation system to induce and evaluate the stem cell differentiation process under given physical stimulations. In this system, all the driving signals of physical stimulations can be controlled independently by a reprogrammable microcomputer for physical stimulation studies. This chapter focuses on the design methodology and characterization of this cell culture system.