Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes.

Yu Tin Chen, Hwei Ling Peng, Wei Chung Shia, Fang Rong Hsu, Chuian-Fu Ken, Yu Ming Tsao, Chang Hua Chen, Chun Eng Liu, Ming Feng Hsieh, Huang Chi Chen, Chuan Yi Tang, Tien Hsiung Ku

Research output: Contribution to journalArticle

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Abstract

The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria. The 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15% and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii. The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use (eut operon) and cobalamin (vitamin B12) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non-Proteeae enterobacterial genomes. This is the first genome sequence of M. morganii, which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae. Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen.

Original languageEnglish
JournalUnknown Journal
Volume13 Suppl 7
Publication statusPublished - 2012 Jan 1

Fingerprint

Morganella morganii
Virulence
Genome
Genes
Vitamin B 12
Operon
IgA-specific serine endopeptidase
Enterobacteriaceae
Bacteremia
Sepsis
Infection
Bacterial Adhesins
Transposases
Bacteriuria
Proteins
Hemolysin Proteins
Ethanolamine
Urease
Postoperative Care
Base Composition

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Genetics

Cite this

Chen, Y. T., Peng, H. L., Shia, W. C., Hsu, F. R., Ken, C-F., Tsao, Y. M., ... Ku, T. H. (2012). Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes. Unknown Journal, 13 Suppl 7.
Chen, Yu Tin ; Peng, Hwei Ling ; Shia, Wei Chung ; Hsu, Fang Rong ; Ken, Chuian-Fu ; Tsao, Yu Ming ; Chen, Chang Hua ; Liu, Chun Eng ; Hsieh, Ming Feng ; Chen, Huang Chi ; Tang, Chuan Yi ; Ku, Tien Hsiung. / Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes. In: Unknown Journal. 2012 ; Vol. 13 Suppl 7.
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abstract = "The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria. The 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15{\%} and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii. The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use (eut operon) and cobalamin (vitamin B12) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non-Proteeae enterobacterial genomes. This is the first genome sequence of M. morganii, which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae. Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen.",
author = "Chen, {Yu Tin} and Peng, {Hwei Ling} and Shia, {Wei Chung} and Hsu, {Fang Rong} and Chuian-Fu Ken and Tsao, {Yu Ming} and Chen, {Chang Hua} and Liu, {Chun Eng} and Hsieh, {Ming Feng} and Chen, {Huang Chi} and Tang, {Chuan Yi} and Ku, {Tien Hsiung}",
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Chen, YT, Peng, HL, Shia, WC, Hsu, FR, Ken, C-F, Tsao, YM, Chen, CH, Liu, CE, Hsieh, MF, Chen, HC, Tang, CY & Ku, TH 2012, 'Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes.', Unknown Journal, vol. 13 Suppl 7.

Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes. / Chen, Yu Tin; Peng, Hwei Ling; Shia, Wei Chung; Hsu, Fang Rong; Ken, Chuian-Fu; Tsao, Yu Ming; Chen, Chang Hua; Liu, Chun Eng; Hsieh, Ming Feng; Chen, Huang Chi; Tang, Chuan Yi; Ku, Tien Hsiung.

In: Unknown Journal, Vol. 13 Suppl 7, 01.01.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes.

AU - Chen, Yu Tin

AU - Peng, Hwei Ling

AU - Shia, Wei Chung

AU - Hsu, Fang Rong

AU - Ken, Chuian-Fu

AU - Tsao, Yu Ming

AU - Chen, Chang Hua

AU - Liu, Chun Eng

AU - Hsieh, Ming Feng

AU - Chen, Huang Chi

AU - Tang, Chuan Yi

AU - Ku, Tien Hsiung

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria. The 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15% and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii. The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use (eut operon) and cobalamin (vitamin B12) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non-Proteeae enterobacterial genomes. This is the first genome sequence of M. morganii, which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae. Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen.

AB - The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria. The 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15% and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii. The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use (eut operon) and cobalamin (vitamin B12) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non-Proteeae enterobacterial genomes. This is the first genome sequence of M. morganii, which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae. Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen.

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