Abstract
We have demonstrated the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vitro and in vivo. We conducted two-channel detection to measure fluorescence at two distinct wavelengths simultaneously. Because the scattering and absorption problems from whole blood were circumvented by the fiber probe, the detected signal strength from the cells were found to be similar in PBS and in whole blood. We achieved the same detection efficiency of the membrane-binding lipophilic dye DiD labeled cells in PBS and in whole blood. High detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was demonstrated. DiD-labeled untransfected and GFPtransfected cells were injected into live mice and the circulation dynamics of the externally injected cells were monitored. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed in whole blood.
| Original language | English |
|---|---|
| Article number | 71730I |
| Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 7173 |
| DOIs | |
| Publication status | Published - 2009 May 5 |
| Event | Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX - San Jose, CA, United States Duration: 2009 Jan 24 → 2009 Jan 25 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
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Two-photon in vivo flow cytometry using a fiber probe. / Chang, Yu-Chung; Ye, Jing Yong; Thomas, Thommey P.; Cao, Zhengyi; Kotlyar, Alina; Tkaczyk, Eric R.; Baker, James R.; Norris, Theodore B.
In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 7173, 71730I, 05.05.2009.Research output: Contribution to journal › Conference article
TY - JOUR
T1 - Two-photon in vivo flow cytometry using a fiber probe
AU - Chang, Yu-Chung
AU - Ye, Jing Yong
AU - Thomas, Thommey P.
AU - Cao, Zhengyi
AU - Kotlyar, Alina
AU - Tkaczyk, Eric R.
AU - Baker, James R.
AU - Norris, Theodore B.
PY - 2009/5/5
Y1 - 2009/5/5
N2 - We have demonstrated the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vitro and in vivo. We conducted two-channel detection to measure fluorescence at two distinct wavelengths simultaneously. Because the scattering and absorption problems from whole blood were circumvented by the fiber probe, the detected signal strength from the cells were found to be similar in PBS and in whole blood. We achieved the same detection efficiency of the membrane-binding lipophilic dye DiD labeled cells in PBS and in whole blood. High detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was demonstrated. DiD-labeled untransfected and GFPtransfected cells were injected into live mice and the circulation dynamics of the externally injected cells were monitored. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed in whole blood.
AB - We have demonstrated the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vitro and in vivo. We conducted two-channel detection to measure fluorescence at two distinct wavelengths simultaneously. Because the scattering and absorption problems from whole blood were circumvented by the fiber probe, the detected signal strength from the cells were found to be similar in PBS and in whole blood. We achieved the same detection efficiency of the membrane-binding lipophilic dye DiD labeled cells in PBS and in whole blood. High detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was demonstrated. DiD-labeled untransfected and GFPtransfected cells were injected into live mice and the circulation dynamics of the externally injected cells were monitored. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed in whole blood.
UR - http://www.scopus.com/inward/record.url?scp=65349135890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349135890&partnerID=8YFLogxK
U2 - 10.1117/12.808436
DO - 10.1117/12.808436
M3 - Conference article
AN - SCOPUS:65349135890
VL - 7173
JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
SN - 1605-7422
M1 - 71730I
ER -