Two-photon in vivo flow cytometry using a fiber probe

Yu-Chung Chang, Jing Yong Ye, Thommey P. Thomas, Zhengyi Cao, Alina Kotlyar, Eric R. Tkaczyk, James R. Baker, Theodore B. Norris

Research output: Contribution to journalConference article

3 Citations (Scopus)

Abstract

We have demonstrated the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vitro and in vivo. We conducted two-channel detection to measure fluorescence at two distinct wavelengths simultaneously. Because the scattering and absorption problems from whole blood were circumvented by the fiber probe, the detected signal strength from the cells were found to be similar in PBS and in whole blood. We achieved the same detection efficiency of the membrane-binding lipophilic dye DiD labeled cells in PBS and in whole blood. High detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was demonstrated. DiD-labeled untransfected and GFPtransfected cells were injected into live mice and the circulation dynamics of the externally injected cells were monitored. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed in whole blood.

Original languageEnglish
Article number71730I
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7173
DOIs
Publication statusPublished - 2009 May 5
EventOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX - San Jose, CA, United States
Duration: 2009 Jan 242009 Jan 25

Fingerprint

cytometry
Flow cytometry
Photons
Flow Cytometry
Blood
blood
fibers
Fibers
probes
photons
cells
Green Fluorescent Proteins
Proteins
proteins
Coloring Agents
Dyes
Fluorescence
mice
Scattering
Membranes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Chang, Y-C., Ye, J. Y., Thomas, T. P., Cao, Z., Kotlyar, A., Tkaczyk, E. R., ... Norris, T. B. (2009). Two-photon in vivo flow cytometry using a fiber probe. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7173, [71730I]. https://doi.org/10.1117/12.808436
Chang, Yu-Chung ; Ye, Jing Yong ; Thomas, Thommey P. ; Cao, Zhengyi ; Kotlyar, Alina ; Tkaczyk, Eric R. ; Baker, James R. ; Norris, Theodore B. / Two-photon in vivo flow cytometry using a fiber probe. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2009 ; Vol. 7173.
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Chang, Y-C, Ye, JY, Thomas, TP, Cao, Z, Kotlyar, A, Tkaczyk, ER, Baker, JR & Norris, TB 2009, 'Two-photon in vivo flow cytometry using a fiber probe', Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7173, 71730I. https://doi.org/10.1117/12.808436

Two-photon in vivo flow cytometry using a fiber probe. / Chang, Yu-Chung; Ye, Jing Yong; Thomas, Thommey P.; Cao, Zhengyi; Kotlyar, Alina; Tkaczyk, Eric R.; Baker, James R.; Norris, Theodore B.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 7173, 71730I, 05.05.2009.

Research output: Contribution to journalConference article

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AU - Norris, Theodore B.

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