Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber

Yu-Chung Chang, Jing Yong Ye, Thommey Thomas, Yi Chen, James R. Baker, Theodore B. Norris

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We report on the utilization of a dual-clad optical fiber for two-photon excited fluorescence correlation spectroscopy (FCS). High excitation efficiency is obtained by sending prechirped ultrafast pulses through the single-mode core of the fiber, while the fluorescence collection efficiency is enhanced because of the larger numerical aperture of the inner cladding. We show that the utilization of a dual-clad fiber is critical for ultrasensitive two-photon fluorescence detection. Our system has the ability to detect fluorescent nanospheres as small as 12 nm in radius. Quantum dots of radius 7 nm are also measured and show excellent signal to noise ratio. The particle sizes obtained from the fiber FCS system were confirmed by measurements using a commercial dynamic light scattering (DLS) system.

Original languageEnglish
Pages (from-to)12640-12649
Number of pages10
JournalOptics Express
Volume16
Issue number17
DOIs
Publication statusPublished - 2008 Aug 18

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optical fibers
fluorescence
photons
spectroscopy
fibers
radii
numerical aperture
signal to noise ratios
light scattering
quantum dots
pulses
excitation

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Chang, Y-C., Ye, J. Y., Thomas, T., Chen, Y., Baker, J. R., & Norris, T. B. (2008). Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber. Optics Express, 16(17), 12640-12649. https://doi.org/10.1364/OE.16.012640
Chang, Yu-Chung ; Ye, Jing Yong ; Thomas, Thommey ; Chen, Yi ; Baker, James R. ; Norris, Theodore B. / Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber. In: Optics Express. 2008 ; Vol. 16, No. 17. pp. 12640-12649.
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Chang, Y-C, Ye, JY, Thomas, T, Chen, Y, Baker, JR & Norris, TB 2008, 'Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber', Optics Express, vol. 16, no. 17, pp. 12640-12649. https://doi.org/10.1364/OE.16.012640

Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber. / Chang, Yu-Chung; Ye, Jing Yong; Thomas, Thommey; Chen, Yi; Baker, James R.; Norris, Theodore B.

In: Optics Express, Vol. 16, No. 17, 18.08.2008, p. 12640-12649.

Research output: Contribution to journalArticle

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