Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis

Shih Feng Fu, Po Yu Chen, Quynh Thi T. Nguyen, Li Yao Huang, Guan Ru Zeng, Tsai Lien Huang, Chung Yi Lin, Hao Jen Huang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Arsenic (As) is a toxic metalloid found ubiquitously in the environment and widely considered an acute poison and carcinogen. However, the molecular mechanisms of the plant response to As and ensuing tolerance have not been extensively characterized. Here, we report on transcriptional changes with As treatment in two Arabidopsis accessions, Col-0 and Ws-2.Results: The root elongation rate was greater for Col-0 than Ws-2 with As exposure. Accumulation of As was lower in the more tolerant accession Col-0 than in Ws-2. We compared the effect of As exposure on genome-wide gene expression in the two accessions by comparative microarray assay. The genes related to heat response and oxidative stresses were common to both accessions, which indicates conserved As stress-associated responses for the two accessions. Most of the specific response genes encoded heat shock proteins, heat shock factors, ubiquitin and aquaporin transporters. Genes coding for ethylene-signalling components were enriched in As-tolerant Col-0 with As exposure. A tolerance-associated gene candidate encoding Leucine-Rich Repeat receptor-like kinase VIII (LRR-RLK VIII) was selected for functional characterization. Genetic loss-of-function analysis of the LRR-RLK VIII gene revealed altered As sensitivity and the metal accumulation in roots.Conclusions: Thus, ethylene-related pathways, maintenance of protein structure and LRR-RLK VIII-mediated signalling may be important mechanisms for toxicity and tolerance to As in the species. Here, we provide a comprehensive survey of global transcriptional regulation for As and identify stress- and tolerance-associated genes responding to As.

Original languageEnglish
Article number94
JournalBMC Plant Biology
Volume14
Issue number1
DOIs
Publication statusPublished - 2014 Apr 16

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arsenic
transcriptomics
Arabidopsis
toxicity
genes
leucine
phosphotransferases (kinases)
receptors
ethylene
aquaporins
carcinogens
protein structure
ubiquitin
heat shock proteins
heat stress
transporters
plant response
root growth
oxidative stress
metals

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Fu, S. F., Chen, P. Y., Nguyen, Q. T. T., Huang, L. Y., Zeng, G. R., Huang, T. L., ... Huang, H. J. (2014). Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis. BMC Plant Biology, 14(1), [94]. https://doi.org/10.1186/1471-2229-14-94
Fu, Shih Feng ; Chen, Po Yu ; Nguyen, Quynh Thi T. ; Huang, Li Yao ; Zeng, Guan Ru ; Huang, Tsai Lien ; Lin, Chung Yi ; Huang, Hao Jen. / Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis. In: BMC Plant Biology. 2014 ; Vol. 14, No. 1.
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abstract = "Background: Arsenic (As) is a toxic metalloid found ubiquitously in the environment and widely considered an acute poison and carcinogen. However, the molecular mechanisms of the plant response to As and ensuing tolerance have not been extensively characterized. Here, we report on transcriptional changes with As treatment in two Arabidopsis accessions, Col-0 and Ws-2.Results: The root elongation rate was greater for Col-0 than Ws-2 with As exposure. Accumulation of As was lower in the more tolerant accession Col-0 than in Ws-2. We compared the effect of As exposure on genome-wide gene expression in the two accessions by comparative microarray assay. The genes related to heat response and oxidative stresses were common to both accessions, which indicates conserved As stress-associated responses for the two accessions. Most of the specific response genes encoded heat shock proteins, heat shock factors, ubiquitin and aquaporin transporters. Genes coding for ethylene-signalling components were enriched in As-tolerant Col-0 with As exposure. A tolerance-associated gene candidate encoding Leucine-Rich Repeat receptor-like kinase VIII (LRR-RLK VIII) was selected for functional characterization. Genetic loss-of-function analysis of the LRR-RLK VIII gene revealed altered As sensitivity and the metal accumulation in roots.Conclusions: Thus, ethylene-related pathways, maintenance of protein structure and LRR-RLK VIII-mediated signalling may be important mechanisms for toxicity and tolerance to As in the species. Here, we provide a comprehensive survey of global transcriptional regulation for As and identify stress- and tolerance-associated genes responding to As.",
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Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis. / Fu, Shih Feng; Chen, Po Yu; Nguyen, Quynh Thi T.; Huang, Li Yao; Zeng, Guan Ru; Huang, Tsai Lien; Lin, Chung Yi; Huang, Hao Jen.

In: BMC Plant Biology, Vol. 14, No. 1, 94, 16.04.2014.

Research output: Contribution to journalArticle

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T1 - Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis

AU - Fu, Shih Feng

AU - Chen, Po Yu

AU - Nguyen, Quynh Thi T.

AU - Huang, Li Yao

AU - Zeng, Guan Ru

AU - Huang, Tsai Lien

AU - Lin, Chung Yi

AU - Huang, Hao Jen

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N2 - Background: Arsenic (As) is a toxic metalloid found ubiquitously in the environment and widely considered an acute poison and carcinogen. However, the molecular mechanisms of the plant response to As and ensuing tolerance have not been extensively characterized. Here, we report on transcriptional changes with As treatment in two Arabidopsis accessions, Col-0 and Ws-2.Results: The root elongation rate was greater for Col-0 than Ws-2 with As exposure. Accumulation of As was lower in the more tolerant accession Col-0 than in Ws-2. We compared the effect of As exposure on genome-wide gene expression in the two accessions by comparative microarray assay. The genes related to heat response and oxidative stresses were common to both accessions, which indicates conserved As stress-associated responses for the two accessions. Most of the specific response genes encoded heat shock proteins, heat shock factors, ubiquitin and aquaporin transporters. Genes coding for ethylene-signalling components were enriched in As-tolerant Col-0 with As exposure. A tolerance-associated gene candidate encoding Leucine-Rich Repeat receptor-like kinase VIII (LRR-RLK VIII) was selected for functional characterization. Genetic loss-of-function analysis of the LRR-RLK VIII gene revealed altered As sensitivity and the metal accumulation in roots.Conclusions: Thus, ethylene-related pathways, maintenance of protein structure and LRR-RLK VIII-mediated signalling may be important mechanisms for toxicity and tolerance to As in the species. Here, we provide a comprehensive survey of global transcriptional regulation for As and identify stress- and tolerance-associated genes responding to As.

AB - Background: Arsenic (As) is a toxic metalloid found ubiquitously in the environment and widely considered an acute poison and carcinogen. However, the molecular mechanisms of the plant response to As and ensuing tolerance have not been extensively characterized. Here, we report on transcriptional changes with As treatment in two Arabidopsis accessions, Col-0 and Ws-2.Results: The root elongation rate was greater for Col-0 than Ws-2 with As exposure. Accumulation of As was lower in the more tolerant accession Col-0 than in Ws-2. We compared the effect of As exposure on genome-wide gene expression in the two accessions by comparative microarray assay. The genes related to heat response and oxidative stresses were common to both accessions, which indicates conserved As stress-associated responses for the two accessions. Most of the specific response genes encoded heat shock proteins, heat shock factors, ubiquitin and aquaporin transporters. Genes coding for ethylene-signalling components were enriched in As-tolerant Col-0 with As exposure. A tolerance-associated gene candidate encoding Leucine-Rich Repeat receptor-like kinase VIII (LRR-RLK VIII) was selected for functional characterization. Genetic loss-of-function analysis of the LRR-RLK VIII gene revealed altered As sensitivity and the metal accumulation in roots.Conclusions: Thus, ethylene-related pathways, maintenance of protein structure and LRR-RLK VIII-mediated signalling may be important mechanisms for toxicity and tolerance to As in the species. Here, we provide a comprehensive survey of global transcriptional regulation for As and identify stress- and tolerance-associated genes responding to As.

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