Transcriptome analysis of age-related gain of callus-forming capacity in arabidopsis hypocotyls

Chi Chien Chen, Shih-Feng Fu, Yung I. Lee, Chung Yi Lin, Wan Chen Lin, Hao Jen Huang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Callus-forming capacity is enhanced with hypocotyl maturity in Arabidopsis. However, the genetic regulation of age-related gain in capacity for callus formation is unclear. We used a gene expression microarray assay to characterize the underlying mechanisms during callus formation in young and mature hypocotyl explants of Arabidopsis. As expected, genes involved in photosynthesis and cell wall thickening showed altered expression during hypocotyl maturation. In addition, genes involved in cytokinin perception were enriched in mature hypocotyl tissues. Phytohormone-induced callus formation in hypocotyl explants was accompanied by increased expression of genes mainly related to the cell cycle, histones and epigenetics. The induction level of these genes was higher in mature hypocotyl explants than young explants during callus formation. We identified a number of genes, including those with unknown function, potentially involved in age-related gain in callus formation. Our results provide insight into the effect of hypocotyl age on callus formation. Altered cytokinin signaling components, cell cycle regulation and epigenetics may work in concert to lead to gain of callus-forming capacity in hypocotyls with age.

Original languageEnglish
Pages (from-to)1457-1469
Number of pages13
JournalPlant and Cell Physiology
Volume53
Issue number8
DOIs
Publication statusPublished - 2012 Aug 1

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Hypocotyl
Bony Callus
Gene Expression Profiling
transcriptomics
Arabidopsis
hypocotyls
callus formation
callus
explants
Cytokinins
Epigenomics
epigenetics
cytokinins
Genes
cell cycle
Cell Cycle
genes
Gene Expression
gene expression
Plant Growth Regulators

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Chen, Chi Chien ; Fu, Shih-Feng ; Lee, Yung I. ; Lin, Chung Yi ; Lin, Wan Chen ; Huang, Hao Jen. / Transcriptome analysis of age-related gain of callus-forming capacity in arabidopsis hypocotyls. In: Plant and Cell Physiology. 2012 ; Vol. 53, No. 8. pp. 1457-1469.
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Transcriptome analysis of age-related gain of callus-forming capacity in arabidopsis hypocotyls. / Chen, Chi Chien; Fu, Shih-Feng; Lee, Yung I.; Lin, Chung Yi; Lin, Wan Chen; Huang, Hao Jen.

In: Plant and Cell Physiology, Vol. 53, No. 8, 01.08.2012, p. 1457-1469.

Research output: Contribution to journalArticle

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