The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): Genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae

Dadbeh Rouhbakhsh, Chi-Yung Lai, Carol D. Von Dohlen, Marta A. Clark, Linda Baumann, Paul Baumann, Nancy A. Moran, David J. Voegtlin

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The bacterial endosymbionts (Buchnera) from the aphids Rhopalosiphum padi, R. maidis, Schizaphis graminum, and Acyrthosiphon pisum contain the genes for anthranilate synthase (trpEG) on plasmids made up of one or more 3.6-kb units. Anthranilate synthase is the first as well as the rate-limiting enzyme in the tryptophan biosynthetic pathway. The amplification of trpEG on plasmids may result in an increase of enzyme protein and overproduction of this essential amino acid, which is required by the aphid host. The nucleotide sequence of trpEG from endosymbionts of different species of aphids is highly conserved, as is an approximately 500-bp upstream DNA segment which has the characteristics of an origin of replication. Phylogenetic analyses were performed using trpE and trpG from the endosymbionts of these four aphids as well as from the endosymbiont of Schlechtendalia chinensis, in which trpEG occurs on the chromosome. The resulting phylogeny was congruent with trees derived from sequences of two chromosome-located bacterial genes (part of trpB and 16S ribosomal DNA). In turn, trees obtained from plasmid-borne and bacterial chromosome-borne sequences were congruent with the tree resulting from phylogenetic analysis of three aphid mitochondrial regions (portions of the small and large ribosomal DNA subunits, as well as cytochrome oxidase II). Congruence of trees based on genes from host mitochondria and from bacteria adds to previous support for exclusively vertical transmission of the endosymbionts within aphid lineages. Congruence with trees based on plasmid-borne genes supports the origin of the plasmid-borne trpEG from the chromosomal genes of the same lineage and the absence of subsequent plasmid exchange among endosymbionts of different species of aphids.

Original languageEnglish
Pages (from-to)414-421
Number of pages8
JournalJournal of Molecular Evolution
Volume42
Issue number4
DOIs
Publication statusPublished - 1996 Jan 1

Fingerprint

Buchnera
Anthranilate Synthase
anthranilate synthase
Aphids
endosymbiont
Molecular Evolution
Biosynthetic Pathways
endosymbionts
Aphididae
aphid
plasmid
Tryptophan
tryptophan
biochemical pathways
Aphidoidea
plasmids
Plasmids
gene
chromosome
chromosomes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Rouhbakhsh, Dadbeh ; Lai, Chi-Yung ; Von Dohlen, Carol D. ; Clark, Marta A. ; Baumann, Linda ; Baumann, Paul ; Moran, Nancy A. ; Voegtlin, David J. / The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera) : Genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae. In: Journal of Molecular Evolution. 1996 ; Vol. 42, No. 4. pp. 414-421.
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abstract = "The bacterial endosymbionts (Buchnera) from the aphids Rhopalosiphum padi, R. maidis, Schizaphis graminum, and Acyrthosiphon pisum contain the genes for anthranilate synthase (trpEG) on plasmids made up of one or more 3.6-kb units. Anthranilate synthase is the first as well as the rate-limiting enzyme in the tryptophan biosynthetic pathway. The amplification of trpEG on plasmids may result in an increase of enzyme protein and overproduction of this essential amino acid, which is required by the aphid host. The nucleotide sequence of trpEG from endosymbionts of different species of aphids is highly conserved, as is an approximately 500-bp upstream DNA segment which has the characteristics of an origin of replication. Phylogenetic analyses were performed using trpE and trpG from the endosymbionts of these four aphids as well as from the endosymbiont of Schlechtendalia chinensis, in which trpEG occurs on the chromosome. The resulting phylogeny was congruent with trees derived from sequences of two chromosome-located bacterial genes (part of trpB and 16S ribosomal DNA). In turn, trees obtained from plasmid-borne and bacterial chromosome-borne sequences were congruent with the tree resulting from phylogenetic analysis of three aphid mitochondrial regions (portions of the small and large ribosomal DNA subunits, as well as cytochrome oxidase II). Congruence of trees based on genes from host mitochondria and from bacteria adds to previous support for exclusively vertical transmission of the endosymbionts within aphid lineages. Congruence with trees based on plasmid-borne genes supports the origin of the plasmid-borne trpEG from the chromosomal genes of the same lineage and the absence of subsequent plasmid exchange among endosymbionts of different species of aphids.",
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The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera) : Genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae. / Rouhbakhsh, Dadbeh; Lai, Chi-Yung; Von Dohlen, Carol D.; Clark, Marta A.; Baumann, Linda; Baumann, Paul; Moran, Nancy A.; Voegtlin, David J.

In: Journal of Molecular Evolution, Vol. 42, No. 4, 01.01.1996, p. 414-421.

Research output: Contribution to journalArticle

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AU - Rouhbakhsh, Dadbeh

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