The optical properties of the silver clusters and their applications in the conformational studies of human telomeric DNA

Hung Chi Hsu, Yang Xiu Lin, Chih Wei Chang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, we investigated the optical properties of the silver nanoclusters (AgNCs) labeled on the 22 bases human telomeric DNA sequences (d[AG3(T2AG3)3], Hum 22AG). The steady state spectra indicate that the formation of the G-quadruplex structure will quench the fluorescence of the AgNCs. In contrast to the AgNCs labeled on the Hum 22AG, the absorption and emission spectra of the AgNCs labeled on the duplex structure are shifted due to the change of local environment and the cluster-base interactions. Moreover, the AgNCs also represent slower spectral relaxation dynamics in the duplex structure, and the result is associated with the stronger hydrogen bond networking and the higher structural rigidity of the duplex. By comparing fluorescence anisotropy dynamics of the Hum 22AG probed by different fluorescence probes, we found that labelling the AgNCs on the DNA would suppress the wobbling motion of the chromophore. The fluorescence anisotropy study also showed an increase in the structural rigidity of the duplex structure.

Original languageEnglish
Pages (from-to)420-424
Number of pages5
JournalDyes and Pigments
Volume146
DOIs
Publication statusPublished - 2017 Nov

Fingerprint

Silver
DNA
Optical properties
Fluorescence
Rigidity
Anisotropy
Nanoclusters
DNA sequences
Chromophores
Labeling
Hydrogen bonds

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Process Chemistry and Technology

Cite this

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title = "The optical properties of the silver clusters and their applications in the conformational studies of human telomeric DNA",
abstract = "In this study, we investigated the optical properties of the silver nanoclusters (AgNCs) labeled on the 22 bases human telomeric DNA sequences (d[AG3(T2AG3)3], Hum 22AG). The steady state spectra indicate that the formation of the G-quadruplex structure will quench the fluorescence of the AgNCs. In contrast to the AgNCs labeled on the Hum 22AG, the absorption and emission spectra of the AgNCs labeled on the duplex structure are shifted due to the change of local environment and the cluster-base interactions. Moreover, the AgNCs also represent slower spectral relaxation dynamics in the duplex structure, and the result is associated with the stronger hydrogen bond networking and the higher structural rigidity of the duplex. By comparing fluorescence anisotropy dynamics of the Hum 22AG probed by different fluorescence probes, we found that labelling the AgNCs on the DNA would suppress the wobbling motion of the chromophore. The fluorescence anisotropy study also showed an increase in the structural rigidity of the duplex structure.",
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The optical properties of the silver clusters and their applications in the conformational studies of human telomeric DNA. / Hsu, Hung Chi; Lin, Yang Xiu; Chang, Chih Wei.

In: Dyes and Pigments, Vol. 146, 11.2017, p. 420-424.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Hsu, Hung Chi

AU - Lin, Yang Xiu

AU - Chang, Chih Wei

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N2 - In this study, we investigated the optical properties of the silver nanoclusters (AgNCs) labeled on the 22 bases human telomeric DNA sequences (d[AG3(T2AG3)3], Hum 22AG). The steady state spectra indicate that the formation of the G-quadruplex structure will quench the fluorescence of the AgNCs. In contrast to the AgNCs labeled on the Hum 22AG, the absorption and emission spectra of the AgNCs labeled on the duplex structure are shifted due to the change of local environment and the cluster-base interactions. Moreover, the AgNCs also represent slower spectral relaxation dynamics in the duplex structure, and the result is associated with the stronger hydrogen bond networking and the higher structural rigidity of the duplex. By comparing fluorescence anisotropy dynamics of the Hum 22AG probed by different fluorescence probes, we found that labelling the AgNCs on the DNA would suppress the wobbling motion of the chromophore. The fluorescence anisotropy study also showed an increase in the structural rigidity of the duplex structure.

AB - In this study, we investigated the optical properties of the silver nanoclusters (AgNCs) labeled on the 22 bases human telomeric DNA sequences (d[AG3(T2AG3)3], Hum 22AG). The steady state spectra indicate that the formation of the G-quadruplex structure will quench the fluorescence of the AgNCs. In contrast to the AgNCs labeled on the Hum 22AG, the absorption and emission spectra of the AgNCs labeled on the duplex structure are shifted due to the change of local environment and the cluster-base interactions. Moreover, the AgNCs also represent slower spectral relaxation dynamics in the duplex structure, and the result is associated with the stronger hydrogen bond networking and the higher structural rigidity of the duplex. By comparing fluorescence anisotropy dynamics of the Hum 22AG probed by different fluorescence probes, we found that labelling the AgNCs on the DNA would suppress the wobbling motion of the chromophore. The fluorescence anisotropy study also showed an increase in the structural rigidity of the duplex structure.

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