In the conception of stories, most beginners create stories at their will, which would result in a poor architecture. At present, few digital storytelling systems have been developed to lead beginners to stories featuring a complete structure. With emphasis on story structure, this study aims to develop a digital storytelling system, Digital Storytelling Teaching System-University (DSTS-U). With the system, learners will be able to quickly create stories with a structural architecture and enhance the variation of the contents of stories through different story structures. Moreover, this study probes into the usability of the system from three dimensions, namely, teaching environment assessment, system features evaluation, and self-story assessment. The subjects of this study were 40 college students who were all beginners, including 21 males and 19 females. The experiment lasted for four weeks. According to the research findings, the experts made positive comments on the teaching environment the DSTS-U system provided for learners. This indicates that learners can create a structural story and acquire more knowledge of story concept with the DSTS-U system. Additionally, positive comments were also made on user’s system function features evaluation as well as on self-story satisfaction. For users, the DSTS-U system is an efficient tool to develop stories in a profound way. Therefore, this study has come to the conclusion that the story structure-led teaching model is necessary. By acquiring the knowledge of the structural story concept, learners can generate a structural story from a basic structural architecture with the help of different variation-oriented structures, so as to enrich the contents of stories.