This paper aims to explore the theories and interrelationships of the effectiveness of creating e-portfolios for enhancing the reflection and aesthetic literacy of first-year college students. First, the paper re-examined the reflection scale and the aesthetic literacy scale with a questionnaire survey of first-year university students in the central of Taiwan. Moreover, this paper justified the relationships between the reflection and aesthetic literacy scales and their dimensions. Furthermore, the research results show that the reflection is highly correlation to the depth of the digital files and aesthetic literacy is highly correlation to the visual presentation of the digital files. Both variables can suitably reflect the student's characteristics in the e-portfolio., which provides a foundation for future research. Finally, the research attempted to encourage the school educational incorporating the use of e-portfolios in the 12-year compulsory education curricula.