Synergistic antitumor effect of curcumin and dinitrosyl iron complexes for against melanoma cells

Yu Der Wen, Yun Lung Ho, Rong Jen Shiau, Jung Kai Yeh, Jheng Yu Wu, Wei Lung Wang, Show Jen Chiou

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The aim of this study was to examine whether combining curcumin, a chemoprevention agent, and dinitrosyl iron complexes (DNICs) would have a synergistic cytotoxic effect on mouse melanoma B16-F10 cells in vitro. Three synthesized DNICs-[PPN] [(NO)2Fe(SCH2CONHCH3)2] (NC01), [PPN] [(NO)2Fe(SCH2CON(CH3)2)2] (NC02), and [Na][(NO)2Fe(SCH2CON(CH3)2)2] (NC03) were tested in this study. In vitro DNA cleavage assay showed all three DNICs could cause plasmid DNA damage through releasing NO under UV irradiation. The cytotoxicity assay demonstrated these DNICs were toxic to B16-F10 cells in vitro, and the estimated values of LD50 (24 h of incubation) of NC01 and NC02 were 1 μM, while the values of LD50 of NC03 was 200 μM. No synergistic cytotoxicity effect was noted in the treatments of the combinations of curcumin and DNICs. On the contrary, in the presence of NC03, the toxicity of curcumin was reduced. Using UV-Visible spectroscopy and fluorescence microscopy, we found NC03 might interact with curcumin and reduce the accumulation of curcumin in cells. Further experiments using the pretreatment of curcumin for 4 h followed by the treatment of NC03 showed the synergistic cytotoxic effect, while, the pretreatment of NC03 followed by the treatment of curcumin did not have any effect. This study provides the basis for further investigation on the effects of combinations of curcumin and other NO donors.

Original languageEnglish
Pages (from-to)352-359
Number of pages8
JournalJournal of Organometallic Chemistry
Volume695
Issue number3
DOIs
Publication statusPublished - 2010 Feb 1

Fingerprint

Curcumin
Melanoma
Iron
iron
cells
Cytotoxicity
pretreatment
Assays
DNA
deoxyribonucleic acid
Lethal Dose 50
plasmids
Fluorescence microscopy
releasing
toxicity
mice
Toxicity
cleavage
DNA Cleavage
Experimental Melanomas

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry
  • Materials Chemistry

Cite this

Wen, Yu Der ; Ho, Yun Lung ; Shiau, Rong Jen ; Yeh, Jung Kai ; Wu, Jheng Yu ; Wang, Wei Lung ; Chiou, Show Jen. / Synergistic antitumor effect of curcumin and dinitrosyl iron complexes for against melanoma cells. In: Journal of Organometallic Chemistry. 2010 ; Vol. 695, No. 3. pp. 352-359.
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Synergistic antitumor effect of curcumin and dinitrosyl iron complexes for against melanoma cells. / Wen, Yu Der; Ho, Yun Lung; Shiau, Rong Jen; Yeh, Jung Kai; Wu, Jheng Yu; Wang, Wei Lung; Chiou, Show Jen.

In: Journal of Organometallic Chemistry, Vol. 695, No. 3, 01.02.2010, p. 352-359.

Research output: Contribution to journalArticle

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T1 - Synergistic antitumor effect of curcumin and dinitrosyl iron complexes for against melanoma cells

AU - Wen, Yu Der

AU - Ho, Yun Lung

AU - Shiau, Rong Jen

AU - Yeh, Jung Kai

AU - Wu, Jheng Yu

AU - Wang, Wei Lung

AU - Chiou, Show Jen

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AB - The aim of this study was to examine whether combining curcumin, a chemoprevention agent, and dinitrosyl iron complexes (DNICs) would have a synergistic cytotoxic effect on mouse melanoma B16-F10 cells in vitro. Three synthesized DNICs-[PPN] [(NO)2Fe(SCH2CONHCH3)2] (NC01), [PPN] [(NO)2Fe(SCH2CON(CH3)2)2] (NC02), and [Na][(NO)2Fe(SCH2CON(CH3)2)2] (NC03) were tested in this study. In vitro DNA cleavage assay showed all three DNICs could cause plasmid DNA damage through releasing NO under UV irradiation. The cytotoxicity assay demonstrated these DNICs were toxic to B16-F10 cells in vitro, and the estimated values of LD50 (24 h of incubation) of NC01 and NC02 were 1 μM, while the values of LD50 of NC03 was 200 μM. No synergistic cytotoxicity effect was noted in the treatments of the combinations of curcumin and DNICs. On the contrary, in the presence of NC03, the toxicity of curcumin was reduced. Using UV-Visible spectroscopy and fluorescence microscopy, we found NC03 might interact with curcumin and reduce the accumulation of curcumin in cells. Further experiments using the pretreatment of curcumin for 4 h followed by the treatment of NC03 showed the synergistic cytotoxic effect, while, the pretreatment of NC03 followed by the treatment of curcumin did not have any effect. This study provides the basis for further investigation on the effects of combinations of curcumin and other NO donors.

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