Simultaneous detection of multiple pathogens by multiplex PCR coupled with DNA biochip hybridization

Hsiang Yun Tung, Wei Chen Chen, Bor Rung Ou, Jan Ying Yeh, Yeong Hsiang Cheng, Ping Hua Tsng, Ming-Hua Hsu, Ming Shiun Tsai, Yu Chuan Liang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Traditional serological enzyme-linked immunosorbent assay (ELISA) is routinely used to monitor pathogens during quarantine in most animal facilities to prevent possible infection. However, the ELISA platform is a single-target assay, and screening all targeted pathogens is time-consuming and laborious. In this study, to increase sensitivity and to reduce diagnosis time for high-throughput processes, multiplex PCR and DNA biochip techniques were combined to develop a multi-pathogen diagnostic method for use instead of routine ELISA. Eight primer sets were designed for multiplex PCR to detect genes from seven targeted bacterial and viral pathogens. DNA–DNA hybridization was conducted on a biochip following the multiple PCR analysis. Using this method, a total of 24 clinical samples were tested, and the result showed that not only single infection but also co-infection by multi-pathogens can be detected. In conclusion, multiplex PCR coupled with a DNA biochip is an efficient method for detecting multi-pathogens in a reaction. This platform is a useful tool for quarantine services and disease prevention in animal facilities.

Original languageEnglish
Pages (from-to)186-195
Number of pages10
JournalLaboratory Animals
Volume52
Issue number2
DOIs
Publication statusPublished - 2018 Apr 1

Fingerprint

Multiplex Polymerase Chain Reaction
nucleic acid hybridization
Quarantine
Enzyme-Linked Immunosorbent Assay
pathogens
DNA
enzyme-linked immunosorbent assay
Infection
quarantine
Coinfection
Polymerase Chain Reaction
disease prevention
mixed infection
infection
diagnostic techniques
Genes
animals
hybridization
methodology
screening

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Tung, H. Y., Chen, W. C., Ou, B. R., Yeh, J. Y., Cheng, Y. H., Tsng, P. H., ... Liang, Y. C. (2018). Simultaneous detection of multiple pathogens by multiplex PCR coupled with DNA biochip hybridization. Laboratory Animals, 52(2), 186-195. https://doi.org/10.1177/0023677217718864
Tung, Hsiang Yun ; Chen, Wei Chen ; Ou, Bor Rung ; Yeh, Jan Ying ; Cheng, Yeong Hsiang ; Tsng, Ping Hua ; Hsu, Ming-Hua ; Tsai, Ming Shiun ; Liang, Yu Chuan. / Simultaneous detection of multiple pathogens by multiplex PCR coupled with DNA biochip hybridization. In: Laboratory Animals. 2018 ; Vol. 52, No. 2. pp. 186-195.
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Tung, HY, Chen, WC, Ou, BR, Yeh, JY, Cheng, YH, Tsng, PH, Hsu, M-H, Tsai, MS & Liang, YC 2018, 'Simultaneous detection of multiple pathogens by multiplex PCR coupled with DNA biochip hybridization', Laboratory Animals, vol. 52, no. 2, pp. 186-195. https://doi.org/10.1177/0023677217718864

Simultaneous detection of multiple pathogens by multiplex PCR coupled with DNA biochip hybridization. / Tung, Hsiang Yun; Chen, Wei Chen; Ou, Bor Rung; Yeh, Jan Ying; Cheng, Yeong Hsiang; Tsng, Ping Hua; Hsu, Ming-Hua; Tsai, Ming Shiun; Liang, Yu Chuan.

In: Laboratory Animals, Vol. 52, No. 2, 01.04.2018, p. 186-195.

Research output: Contribution to journalArticle

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AU - Tsng, Ping Hua

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AU - Tsai, Ming Shiun

AU - Liang, Yu Chuan

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