Simultaneous Analysis of Biogenic Amines in Canned Fish by HPLC

GOW‐CHIN ‐C YEN, Chiu-Lan Hsieh

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95 Citations (Scopus)


A simple HPLC method was developed for simultaneous determination of putrescine, cadaverine, tryptamine, 2‐phenylethylamine, spermidine, spermine, histamine, tyramine, and agmatine. Amines were derivatized with benzoyl chloride and separated well on Lichrosphere are 100 RP‐18 column using a gradient elution system with a mixture of methanol and water in 10 min. Amine content of canned fish was detected by this method, and except for anchovie products most samples contained the nine amines at low levels. This method would be useful for detection of amines in fish.

Original languageEnglish
Pages (from-to)158-160
Number of pages3
JournalJournal of Food Science
Issue number1
Publication statusPublished - 1991 Jan 1

All Science Journal Classification (ASJC) codes

  • Food Science

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