Abstract
These studies investigate if crustacean hyperglycemic hormone (CHH) is involved in 5-hydroxytryptamine (5-HT)-induced hyperglycemia. Eyestalk ganglia with intact X-organ-sinus gland complex were dissected from the crayfish Procambarus clarkii and incubated under various experimental conditions. Incubation media were then analyzed for the presence of released hyperglycemic factor using an in vivo bioassay. The results show that 5-HT enhanced release of hyperglycemic factor in a dose-dependent manner. This stimulatory effect of 5-HT was significantly decreased by adding ketanserin or methysergide (both 5-HT receptor antagonists) into incubation of eye stalk ganglia. Further, activity of the 5-HT-released hyperglycemic factor could be eliminated by adsorption of incubation media with anti-CHH serum but not by preimmune or anti-5-HT serum. These results confirm the hypothesis that 5-HT enhances release of CHH, which in turn elicits hyperglycemic responses. It is probable that 5-HT activates an excitation-secretion coupling mechanism by interacting with receptors located on the X-organ neurosecretory cells.
| Original language | English |
|---|---|
| Pages (from-to) | 376-382 |
| Number of pages | 7 |
| Journal | Physiological and Biochemical Zoology |
| Volume | 74 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2001 Jun 15 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Biochemistry
- Animal Science and Zoology
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Serotonergic regulation of crustacean hyperglycemic hormone secretion in the crayfish, Procambarus clarkii. / Lee, C. Y.; Yang, P. F.; Zou, H. S.
In: Physiological and Biochemical Zoology, Vol. 74, No. 3, 15.06.2001, p. 376-382.Research output: Contribution to journal › Article
TY - JOUR
T1 - Serotonergic regulation of crustacean hyperglycemic hormone secretion in the crayfish, Procambarus clarkii
AU - Lee, C. Y.
AU - Yang, P. F.
AU - Zou, H. S.
PY - 2001/6/15
Y1 - 2001/6/15
N2 - These studies investigate if crustacean hyperglycemic hormone (CHH) is involved in 5-hydroxytryptamine (5-HT)-induced hyperglycemia. Eyestalk ganglia with intact X-organ-sinus gland complex were dissected from the crayfish Procambarus clarkii and incubated under various experimental conditions. Incubation media were then analyzed for the presence of released hyperglycemic factor using an in vivo bioassay. The results show that 5-HT enhanced release of hyperglycemic factor in a dose-dependent manner. This stimulatory effect of 5-HT was significantly decreased by adding ketanserin or methysergide (both 5-HT receptor antagonists) into incubation of eye stalk ganglia. Further, activity of the 5-HT-released hyperglycemic factor could be eliminated by adsorption of incubation media with anti-CHH serum but not by preimmune or anti-5-HT serum. These results confirm the hypothesis that 5-HT enhances release of CHH, which in turn elicits hyperglycemic responses. It is probable that 5-HT activates an excitation-secretion coupling mechanism by interacting with receptors located on the X-organ neurosecretory cells.
AB - These studies investigate if crustacean hyperglycemic hormone (CHH) is involved in 5-hydroxytryptamine (5-HT)-induced hyperglycemia. Eyestalk ganglia with intact X-organ-sinus gland complex were dissected from the crayfish Procambarus clarkii and incubated under various experimental conditions. Incubation media were then analyzed for the presence of released hyperglycemic factor using an in vivo bioassay. The results show that 5-HT enhanced release of hyperglycemic factor in a dose-dependent manner. This stimulatory effect of 5-HT was significantly decreased by adding ketanserin or methysergide (both 5-HT receptor antagonists) into incubation of eye stalk ganglia. Further, activity of the 5-HT-released hyperglycemic factor could be eliminated by adsorption of incubation media with anti-CHH serum but not by preimmune or anti-5-HT serum. These results confirm the hypothesis that 5-HT enhances release of CHH, which in turn elicits hyperglycemic responses. It is probable that 5-HT activates an excitation-secretion coupling mechanism by interacting with receptors located on the X-organ neurosecretory cells.
UR - http://www.scopus.com/inward/record.url?scp=0034991717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034991717&partnerID=8YFLogxK
U2 - 10.1086/320430
DO - 10.1086/320430
M3 - Article
C2 - 11331509
AN - SCOPUS:0034991717
VL - 74
SP - 376
EP - 382
JO - Physiological and Biochemical Zoology
JF - Physiological and Biochemical Zoology
SN - 1522-2152
IS - 3
ER -