Serotonergic regulation of crustacean hyperglycemic hormone secretion in the crayfish, Procambarus clarkii

C. Y. Lee, P. F. Yang, H. S. Zou

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

These studies investigate if crustacean hyperglycemic hormone (CHH) is involved in 5-hydroxytryptamine (5-HT)-induced hyperglycemia. Eyestalk ganglia with intact X-organ-sinus gland complex were dissected from the crayfish Procambarus clarkii and incubated under various experimental conditions. Incubation media were then analyzed for the presence of released hyperglycemic factor using an in vivo bioassay. The results show that 5-HT enhanced release of hyperglycemic factor in a dose-dependent manner. This stimulatory effect of 5-HT was significantly decreased by adding ketanserin or methysergide (both 5-HT receptor antagonists) into incubation of eye stalk ganglia. Further, activity of the 5-HT-released hyperglycemic factor could be eliminated by adsorption of incubation media with anti-CHH serum but not by preimmune or anti-5-HT serum. These results confirm the hypothesis that 5-HT enhances release of CHH, which in turn elicits hyperglycemic responses. It is probable that 5-HT activates an excitation-secretion coupling mechanism by interacting with receptors located on the X-organ neurosecretory cells.

Original languageEnglish
Pages (from-to)376-382
Number of pages7
JournalPhysiological and Biochemical Zoology
Volume74
Issue number3
DOIs
Publication statusPublished - 2001 Jun 15

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Astacoidea
Procambarus clarkii
hormone secretion
crayfish
serotonin
Serotonin
Crustacea
hormones
Ganglia
Methysergide
Ketanserin
neurosecretory cells
Serotonin Antagonists
Bioassay
Serotonin Receptors
hyperglycemia
sinuses
crustacean hyperglycemic hormone
Serum
Hyperglycemia

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Animal Science and Zoology

Cite this

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abstract = "These studies investigate if crustacean hyperglycemic hormone (CHH) is involved in 5-hydroxytryptamine (5-HT)-induced hyperglycemia. Eyestalk ganglia with intact X-organ-sinus gland complex were dissected from the crayfish Procambarus clarkii and incubated under various experimental conditions. Incubation media were then analyzed for the presence of released hyperglycemic factor using an in vivo bioassay. The results show that 5-HT enhanced release of hyperglycemic factor in a dose-dependent manner. This stimulatory effect of 5-HT was significantly decreased by adding ketanserin or methysergide (both 5-HT receptor antagonists) into incubation of eye stalk ganglia. Further, activity of the 5-HT-released hyperglycemic factor could be eliminated by adsorption of incubation media with anti-CHH serum but not by preimmune or anti-5-HT serum. These results confirm the hypothesis that 5-HT enhances release of CHH, which in turn elicits hyperglycemic responses. It is probable that 5-HT activates an excitation-secretion coupling mechanism by interacting with receptors located on the X-organ neurosecretory cells.",
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Serotonergic regulation of crustacean hyperglycemic hormone secretion in the crayfish, Procambarus clarkii. / Lee, C. Y.; Yang, P. F.; Zou, H. S.

In: Physiological and Biochemical Zoology, Vol. 74, No. 3, 15.06.2001, p. 376-382.

Research output: Contribution to journalArticle

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