Serotonergic regulation of blood glucose levels in the crayfish, Procambarus clarkii: Site of action and receptor characterization

Chi-Ying Lee, Shiou Mei Yau, Ching Sheyen Liau, Wen Jr Huang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The present study investigated the site of action of 5-hydroxytryptamine (5-HT) and pharmacologically characterized the receptors involved in regulating blood glucose levels in the crayfish, Procambarus clarkii. Injection of 5-HT into intact animals increased glucose levels in a dose-dependent manner. In contrast, 5-HT failed to elicit a hyperglycemic response in eyestalk-ablated animals. Effects of several 5-HT receptor agonists and antagonists were examined. 5-CT, oxymetazoline (both 5-HT1 receptor agonists) and α-methyl-5-HT (a 5-HT2 receptor agonist), but not 1-phenylbiguanide, m-CPBG (both 5-HT3 receptor agonists), or RS 67333 (a 5-HT4 receptor agonist), induced hyperglycemic responses in a dose-dependent manner. In addition, 8-OH-DPAT (a 5-HT(1A) receptor agonist), L-694,247 (a 5-HT(1B/1D) receptor agonist), and DOI (a 5-HT(2A) receptor agonist) were effective in significantly increasing the glucose levels, whereas both BW 723C86 (a 5-HT(2B) receptor agonist) and m-CPP (a 5-HT(2C) receptor agonist) were ineffective. Finally, ketanserin (a 5-HT(2A) receptor antagonist), but not p-MPPF (a 5-HT(1A) receptor antagonist), GR 55562 (a 5-HT(1B/1D) receptor antagonist), SB 206553 (a 5-HT(2B/2C) receptor antagonist), or tropisetron (a 5-HT3 receptor antagonist), was able to block 5-HT-induced hyperglycemia. The combined results support the hypothesis that 5-HT exerts its hyperglycemic effect by enhancing the release of hyperglycemic factor(s) from the eyestalks, and suggest that 5 HT-induced hyperglycemia is mediated by 5-HT1- and 5-HT2-like receptors. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)596-605
Number of pages10
JournalJournal of Experimental Zoology
Volume286
Issue number6
DOIs
Publication statusPublished - 2000 May 1

Fingerprint

Procambarus clarkii
crayfish
serotonin
blood glucose
receptors
agonists
antagonists
hyperglycemia
glycemic effect
glucose

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

@article{6bb4cc03db7e42e1be91536d636e22dd,
title = "Serotonergic regulation of blood glucose levels in the crayfish, Procambarus clarkii: Site of action and receptor characterization",
abstract = "The present study investigated the site of action of 5-hydroxytryptamine (5-HT) and pharmacologically characterized the receptors involved in regulating blood glucose levels in the crayfish, Procambarus clarkii. Injection of 5-HT into intact animals increased glucose levels in a dose-dependent manner. In contrast, 5-HT failed to elicit a hyperglycemic response in eyestalk-ablated animals. Effects of several 5-HT receptor agonists and antagonists were examined. 5-CT, oxymetazoline (both 5-HT1 receptor agonists) and α-methyl-5-HT (a 5-HT2 receptor agonist), but not 1-phenylbiguanide, m-CPBG (both 5-HT3 receptor agonists), or RS 67333 (a 5-HT4 receptor agonist), induced hyperglycemic responses in a dose-dependent manner. In addition, 8-OH-DPAT (a 5-HT(1A) receptor agonist), L-694,247 (a 5-HT(1B/1D) receptor agonist), and DOI (a 5-HT(2A) receptor agonist) were effective in significantly increasing the glucose levels, whereas both BW 723C86 (a 5-HT(2B) receptor agonist) and m-CPP (a 5-HT(2C) receptor agonist) were ineffective. Finally, ketanserin (a 5-HT(2A) receptor antagonist), but not p-MPPF (a 5-HT(1A) receptor antagonist), GR 55562 (a 5-HT(1B/1D) receptor antagonist), SB 206553 (a 5-HT(2B/2C) receptor antagonist), or tropisetron (a 5-HT3 receptor antagonist), was able to block 5-HT-induced hyperglycemia. The combined results support the hypothesis that 5-HT exerts its hyperglycemic effect by enhancing the release of hyperglycemic factor(s) from the eyestalks, and suggest that 5 HT-induced hyperglycemia is mediated by 5-HT1- and 5-HT2-like receptors. (C) 2000 Wiley-Liss, Inc.",
author = "Chi-Ying Lee and Yau, {Shiou Mei} and Liau, {Ching Sheyen} and Huang, {Wen Jr}",
year = "2000",
month = "5",
day = "1",
doi = "10.1002/(SICI)1097-010X(20000501)286:6<596::AID-JEZ6>3.0.CO;2-S",
language = "English",
volume = "286",
pages = "596--605",
journal = "Journal of Experimental Zoology",
issn = "1548-8969",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

Serotonergic regulation of blood glucose levels in the crayfish, Procambarus clarkii : Site of action and receptor characterization. / Lee, Chi-Ying; Yau, Shiou Mei; Liau, Ching Sheyen; Huang, Wen Jr.

In: Journal of Experimental Zoology, Vol. 286, No. 6, 01.05.2000, p. 596-605.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Serotonergic regulation of blood glucose levels in the crayfish, Procambarus clarkii

T2 - Site of action and receptor characterization

AU - Lee, Chi-Ying

AU - Yau, Shiou Mei

AU - Liau, Ching Sheyen

AU - Huang, Wen Jr

PY - 2000/5/1

Y1 - 2000/5/1

N2 - The present study investigated the site of action of 5-hydroxytryptamine (5-HT) and pharmacologically characterized the receptors involved in regulating blood glucose levels in the crayfish, Procambarus clarkii. Injection of 5-HT into intact animals increased glucose levels in a dose-dependent manner. In contrast, 5-HT failed to elicit a hyperglycemic response in eyestalk-ablated animals. Effects of several 5-HT receptor agonists and antagonists were examined. 5-CT, oxymetazoline (both 5-HT1 receptor agonists) and α-methyl-5-HT (a 5-HT2 receptor agonist), but not 1-phenylbiguanide, m-CPBG (both 5-HT3 receptor agonists), or RS 67333 (a 5-HT4 receptor agonist), induced hyperglycemic responses in a dose-dependent manner. In addition, 8-OH-DPAT (a 5-HT(1A) receptor agonist), L-694,247 (a 5-HT(1B/1D) receptor agonist), and DOI (a 5-HT(2A) receptor agonist) were effective in significantly increasing the glucose levels, whereas both BW 723C86 (a 5-HT(2B) receptor agonist) and m-CPP (a 5-HT(2C) receptor agonist) were ineffective. Finally, ketanserin (a 5-HT(2A) receptor antagonist), but not p-MPPF (a 5-HT(1A) receptor antagonist), GR 55562 (a 5-HT(1B/1D) receptor antagonist), SB 206553 (a 5-HT(2B/2C) receptor antagonist), or tropisetron (a 5-HT3 receptor antagonist), was able to block 5-HT-induced hyperglycemia. The combined results support the hypothesis that 5-HT exerts its hyperglycemic effect by enhancing the release of hyperglycemic factor(s) from the eyestalks, and suggest that 5 HT-induced hyperglycemia is mediated by 5-HT1- and 5-HT2-like receptors. (C) 2000 Wiley-Liss, Inc.

AB - The present study investigated the site of action of 5-hydroxytryptamine (5-HT) and pharmacologically characterized the receptors involved in regulating blood glucose levels in the crayfish, Procambarus clarkii. Injection of 5-HT into intact animals increased glucose levels in a dose-dependent manner. In contrast, 5-HT failed to elicit a hyperglycemic response in eyestalk-ablated animals. Effects of several 5-HT receptor agonists and antagonists were examined. 5-CT, oxymetazoline (both 5-HT1 receptor agonists) and α-methyl-5-HT (a 5-HT2 receptor agonist), but not 1-phenylbiguanide, m-CPBG (both 5-HT3 receptor agonists), or RS 67333 (a 5-HT4 receptor agonist), induced hyperglycemic responses in a dose-dependent manner. In addition, 8-OH-DPAT (a 5-HT(1A) receptor agonist), L-694,247 (a 5-HT(1B/1D) receptor agonist), and DOI (a 5-HT(2A) receptor agonist) were effective in significantly increasing the glucose levels, whereas both BW 723C86 (a 5-HT(2B) receptor agonist) and m-CPP (a 5-HT(2C) receptor agonist) were ineffective. Finally, ketanserin (a 5-HT(2A) receptor antagonist), but not p-MPPF (a 5-HT(1A) receptor antagonist), GR 55562 (a 5-HT(1B/1D) receptor antagonist), SB 206553 (a 5-HT(2B/2C) receptor antagonist), or tropisetron (a 5-HT3 receptor antagonist), was able to block 5-HT-induced hyperglycemia. The combined results support the hypothesis that 5-HT exerts its hyperglycemic effect by enhancing the release of hyperglycemic factor(s) from the eyestalks, and suggest that 5 HT-induced hyperglycemia is mediated by 5-HT1- and 5-HT2-like receptors. (C) 2000 Wiley-Liss, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0034194408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034194408&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-010X(20000501)286:6<596::AID-JEZ6>3.0.CO;2-S

DO - 10.1002/(SICI)1097-010X(20000501)286:6<596::AID-JEZ6>3.0.CO;2-S

M3 - Article

C2 - 10766968

AN - SCOPUS:0034194408

VL - 286

SP - 596

EP - 605

JO - Journal of Experimental Zoology

JF - Journal of Experimental Zoology

SN - 1548-8969

IS - 6

ER -