Serotonergic regulation of blood glucose levels in the crayfish, Procambarus clarkii: Site of action and receptor characterization

Chi Ying Lee, Shiou Mei Yau, Ching Sheyen Liau, Wen Jr Huang

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39 Citations (Scopus)


The present study investigated the site of action of 5-hydroxytryptamine (5-HT) and pharmacologically characterized the receptors involved in regulating blood glucose levels in the crayfish, Procambarus clarkii. Injection of 5-HT into intact animals increased glucose levels in a dose-dependent manner. In contrast, 5-HT failed to elicit a hyperglycemic response in eyestalk-ablated animals. Effects of several 5-HT receptor agonists and antagonists were examined. 5-CT, oxymetazoline (both 5-HT1 receptor agonists) and α-methyl-5-HT (a 5-HT2 receptor agonist), but not 1-phenylbiguanide, m-CPBG (both 5-HT3 receptor agonists), or RS 67333 (a 5-HT4 receptor agonist), induced hyperglycemic responses in a dose-dependent manner. In addition, 8-OH-DPAT (a 5-HT(1A) receptor agonist), L-694,247 (a 5-HT(1B/1D) receptor agonist), and DOI (a 5-HT(2A) receptor agonist) were effective in significantly increasing the glucose levels, whereas both BW 723C86 (a 5-HT(2B) receptor agonist) and m-CPP (a 5-HT(2C) receptor agonist) were ineffective. Finally, ketanserin (a 5-HT(2A) receptor antagonist), but not p-MPPF (a 5-HT(1A) receptor antagonist), GR 55562 (a 5-HT(1B/1D) receptor antagonist), SB 206553 (a 5-HT(2B/2C) receptor antagonist), or tropisetron (a 5-HT3 receptor antagonist), was able to block 5-HT-induced hyperglycemia. The combined results support the hypothesis that 5-HT exerts its hyperglycemic effect by enhancing the release of hyperglycemic factor(s) from the eyestalks, and suggest that 5 HT-induced hyperglycemia is mediated by 5-HT1- and 5-HT2-like receptors. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)596-605
Number of pages10
JournalJournal of Experimental Zoology
Issue number6
Publication statusPublished - 2000 May 1

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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