Abstract
Liquid-phase and solid-phase extractions (SPE) in combination with a simple micellar electrokinetic chromatography (MEKC) method were used to investigate human very low-density lipoprotein (VLDL) lipids for two healthy donors. At absorbance 200 nm, the effective mobilities and peak areas of theMEKC profiles showed good reproducibility and precisions. Amajor peak and several minor peaks appeared for the total lipids of native VLDL, but both the peak numbers and areas reduced for the in vitro oxidized VLDL. Two chloroform and twomethanol fractions were obtained from SPE of VLDLtotal lipids. Significant differences were observed for the first methanol fraction between native and in vitro oxidized VLDL lipids. The first methanol fraction showed a major peak and several minor peaks for native VLDL, but both the peak numbers and areas reduced for oxidized VLDL. Oxidation of VLDL caused decomposition of lipids, and thus the reduction of peak numbers and areas.
| Original language | English |
|---|---|
| Pages (from-to) | 1277-1284 |
| Number of pages | 8 |
| Journal | Journal of the Chinese Chemical Society |
| Volume | 60 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2013 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Separation of lipids on human very low-density lipoproteins by micellar electrokinetic chromatography. / Lai, Wei Ting; Liao, Yi Han; Xie, Huai Guang; Liu, Yi Ning; Lin, Yi Jyun; Liu, Mine-Yine.
In: Journal of the Chinese Chemical Society, Vol. 60, No. 10, 01.01.2013, p. 1277-1284.Research output: Contribution to journal › Article
TY - JOUR
T1 - Separation of lipids on human very low-density lipoproteins by micellar electrokinetic chromatography
AU - Lai, Wei Ting
AU - Liao, Yi Han
AU - Xie, Huai Guang
AU - Liu, Yi Ning
AU - Lin, Yi Jyun
AU - Liu, Mine-Yine
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Liquid-phase and solid-phase extractions (SPE) in combination with a simple micellar electrokinetic chromatography (MEKC) method were used to investigate human very low-density lipoprotein (VLDL) lipids for two healthy donors. At absorbance 200 nm, the effective mobilities and peak areas of theMEKC profiles showed good reproducibility and precisions. Amajor peak and several minor peaks appeared for the total lipids of native VLDL, but both the peak numbers and areas reduced for the in vitro oxidized VLDL. Two chloroform and twomethanol fractions were obtained from SPE of VLDLtotal lipids. Significant differences were observed for the first methanol fraction between native and in vitro oxidized VLDL lipids. The first methanol fraction showed a major peak and several minor peaks for native VLDL, but both the peak numbers and areas reduced for oxidized VLDL. Oxidation of VLDL caused decomposition of lipids, and thus the reduction of peak numbers and areas.
AB - Liquid-phase and solid-phase extractions (SPE) in combination with a simple micellar electrokinetic chromatography (MEKC) method were used to investigate human very low-density lipoprotein (VLDL) lipids for two healthy donors. At absorbance 200 nm, the effective mobilities and peak areas of theMEKC profiles showed good reproducibility and precisions. Amajor peak and several minor peaks appeared for the total lipids of native VLDL, but both the peak numbers and areas reduced for the in vitro oxidized VLDL. Two chloroform and twomethanol fractions were obtained from SPE of VLDLtotal lipids. Significant differences were observed for the first methanol fraction between native and in vitro oxidized VLDL lipids. The first methanol fraction showed a major peak and several minor peaks for native VLDL, but both the peak numbers and areas reduced for oxidized VLDL. Oxidation of VLDL caused decomposition of lipids, and thus the reduction of peak numbers and areas.
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U2 - 10.1002/jccs.201300103
DO - 10.1002/jccs.201300103
M3 - Article
VL - 60
SP - 1277
EP - 1284
JO - Journal of the Chinese Chemical Society
JF - Journal of the Chinese Chemical Society
SN - 0009-4536
IS - 10
ER -