Abstract
A simple capillary zone electrophoresis (CZE) method was used to determine in vitro oxidized phosphatidyl choline (ox-PC). To optimize the capillary electrophoresis (CE) conditions, organic buffer additives, buffer ionic strength, buffer pH, and applied voltage were examined. The optimal CE separation buffer chosen was an aqueous-organic solvent system containing 10% sodium phosphate buffer (5 mM, pH 7.40), 80% methanol, and 10% acetonitrile. One major peak with a small shoulder was found for phosphatidyl choline (PC), whereas one major peak and a complex region containing several lower-mobility peaks were found for ox-PC. The lower-mobility species of ox-PC has high levels of conjugated dienes characterized by strong absorbance at 234 nm. The electropherograms of PC and ox-PC were significantly different and highly reproducible. The intensities of lower-mobility species decreased significantly when the antioxidant vitamin C concentration was increased from 6 to 600 μM. This study provides a simple CZE method to differentiate in vitro oxidized from nonoxidized PC molecular species.
| Original language | English |
|---|---|
| Pages (from-to) | 210-218 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 367 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2007 Aug 15 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Separation and determination of in vitro oxidized phospholipids by capillary zone electrophoresis. / Ho, Yu Ling; Chiu, Jing Huei; Wu, Chung Yu; Liu, Mine Yine.
In: Analytical Biochemistry, Vol. 367, No. 2, 15.08.2007, p. 210-218.Research output: Contribution to journal › Article
TY - JOUR
T1 - Separation and determination of in vitro oxidized phospholipids by capillary zone electrophoresis
AU - Ho, Yu Ling
AU - Chiu, Jing Huei
AU - Wu, Chung Yu
AU - Liu, Mine Yine
PY - 2007/8/15
Y1 - 2007/8/15
N2 - A simple capillary zone electrophoresis (CZE) method was used to determine in vitro oxidized phosphatidyl choline (ox-PC). To optimize the capillary electrophoresis (CE) conditions, organic buffer additives, buffer ionic strength, buffer pH, and applied voltage were examined. The optimal CE separation buffer chosen was an aqueous-organic solvent system containing 10% sodium phosphate buffer (5 mM, pH 7.40), 80% methanol, and 10% acetonitrile. One major peak with a small shoulder was found for phosphatidyl choline (PC), whereas one major peak and a complex region containing several lower-mobility peaks were found for ox-PC. The lower-mobility species of ox-PC has high levels of conjugated dienes characterized by strong absorbance at 234 nm. The electropherograms of PC and ox-PC were significantly different and highly reproducible. The intensities of lower-mobility species decreased significantly when the antioxidant vitamin C concentration was increased from 6 to 600 μM. This study provides a simple CZE method to differentiate in vitro oxidized from nonoxidized PC molecular species.
AB - A simple capillary zone electrophoresis (CZE) method was used to determine in vitro oxidized phosphatidyl choline (ox-PC). To optimize the capillary electrophoresis (CE) conditions, organic buffer additives, buffer ionic strength, buffer pH, and applied voltage were examined. The optimal CE separation buffer chosen was an aqueous-organic solvent system containing 10% sodium phosphate buffer (5 mM, pH 7.40), 80% methanol, and 10% acetonitrile. One major peak with a small shoulder was found for phosphatidyl choline (PC), whereas one major peak and a complex region containing several lower-mobility peaks were found for ox-PC. The lower-mobility species of ox-PC has high levels of conjugated dienes characterized by strong absorbance at 234 nm. The electropherograms of PC and ox-PC were significantly different and highly reproducible. The intensities of lower-mobility species decreased significantly when the antioxidant vitamin C concentration was increased from 6 to 600 μM. This study provides a simple CZE method to differentiate in vitro oxidized from nonoxidized PC molecular species.
UR - http://www.scopus.com/inward/record.url?scp=34347245552&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34347245552&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2007.04.022
DO - 10.1016/j.ab.2007.04.022
M3 - Article
C2 - 17553450
AN - SCOPUS:34347245552
VL - 367
SP - 210
EP - 218
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -