Responses of the arcto-boreal krill species Thysanoessa inermis to variations in water temperature: coupling Hsp70 isoform expressions with metabolism

Kim Huenerlage, Kévin Cascella, Erwan Corre, Lola Toomey, Chi Ying Lee, Friedrich Buchholz, Jean Yves Toullec

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies have indicated a metabolic temperature sensitivity in both the arcto-boreal krill species Thysanoessa inermis and Thysanoessa raschii that may determine these species’ abundance and population persistence at lower latitudes (up to 40° N). T. inermis currently dominates the krill community in the Barents Sea and in the high Arctic Kongsfjord. We aimed to increase the knowledge on the upper thermal limit found in the latter species by estimating the CT50 value (19.7 °C) (critical temperature at which 50 % of animals are reactive) and by linking metabolic rate measurements with molecular approaches. Optical oxygen sensors were used to measure respiration rates in steps of 2 °C (from 0 to 16 °C). To follow the temperature-mediated mechanisms of passive response, i.e., as a proxy for molecular stress, molecular chaperone heat shock protein 70 (Hsp70) sequences were extracted from a transcriptome assembly, and the gene expression kinetics were monitored during an acute temperature exposure to 6 or 10 °C with subsequent recovery at 4 °C. Our results showed upregulation of hsp70 genes, especially the structurally constitutive and mitochondrial isoforms. These findings confirmed the temperature sensitivity of T. inermis and showed that the thermal stress took place before reaching the upper temperature limit estimated by respirometry at 12 °C. This study provides a baseline for further investigations into the thermal tolerances of arcto-boreal Thysanoessa spp. and comparisons with other krill species under different climatic regimes, especially Antarctica.

Original languageEnglish
Pages (from-to)969-981
Number of pages13
JournalCell Stress and Chaperones
Volume21
Issue number6
DOIs
Publication statusPublished - 2016 Nov 1

Fingerprint

Euphausiacea
HSP70 Heat-Shock Proteins
Metabolism
Protein Isoforms
Temperature
Water
Hot Temperature
Oxygen sensors
Molecular Chaperones
Optical sensors
Proxy
Respiratory Rate
Transcriptome
Gene expression
Thermal stress
Oceans and Seas
Animals
Up-Regulation
Genes
Oxygen

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology

Cite this

Huenerlage, Kim ; Cascella, Kévin ; Corre, Erwan ; Toomey, Lola ; Lee, Chi Ying ; Buchholz, Friedrich ; Toullec, Jean Yves. / Responses of the arcto-boreal krill species Thysanoessa inermis to variations in water temperature : coupling Hsp70 isoform expressions with metabolism. In: Cell Stress and Chaperones. 2016 ; Vol. 21, No. 6. pp. 969-981.
@article{131e204803a54e7eadac6d13858d40ec,
title = "Responses of the arcto-boreal krill species Thysanoessa inermis to variations in water temperature: coupling Hsp70 isoform expressions with metabolism",
abstract = "Recent studies have indicated a metabolic temperature sensitivity in both the arcto-boreal krill species Thysanoessa inermis and Thysanoessa raschii that may determine these species’ abundance and population persistence at lower latitudes (up to 40° N). T. inermis currently dominates the krill community in the Barents Sea and in the high Arctic Kongsfjord. We aimed to increase the knowledge on the upper thermal limit found in the latter species by estimating the CT50 value (19.7 °C) (critical temperature at which 50 {\%} of animals are reactive) and by linking metabolic rate measurements with molecular approaches. Optical oxygen sensors were used to measure respiration rates in steps of 2 °C (from 0 to 16 °C). To follow the temperature-mediated mechanisms of passive response, i.e., as a proxy for molecular stress, molecular chaperone heat shock protein 70 (Hsp70) sequences were extracted from a transcriptome assembly, and the gene expression kinetics were monitored during an acute temperature exposure to 6 or 10 °C with subsequent recovery at 4 °C. Our results showed upregulation of hsp70 genes, especially the structurally constitutive and mitochondrial isoforms. These findings confirmed the temperature sensitivity of T. inermis and showed that the thermal stress took place before reaching the upper temperature limit estimated by respirometry at 12 °C. This study provides a baseline for further investigations into the thermal tolerances of arcto-boreal Thysanoessa spp. and comparisons with other krill species under different climatic regimes, especially Antarctica.",
author = "Kim Huenerlage and K{\'e}vin Cascella and Erwan Corre and Lola Toomey and Lee, {Chi Ying} and Friedrich Buchholz and Toullec, {Jean Yves}",
year = "2016",
month = "11",
day = "1",
doi = "10.1007/s12192-016-0720-6",
language = "English",
volume = "21",
pages = "969--981",
journal = "Cell Stress and Chaperones",
issn = "1355-8145",
publisher = "Springer Netherlands",
number = "6",

}

Responses of the arcto-boreal krill species Thysanoessa inermis to variations in water temperature : coupling Hsp70 isoform expressions with metabolism. / Huenerlage, Kim; Cascella, Kévin; Corre, Erwan; Toomey, Lola; Lee, Chi Ying; Buchholz, Friedrich; Toullec, Jean Yves.

In: Cell Stress and Chaperones, Vol. 21, No. 6, 01.11.2016, p. 969-981.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Responses of the arcto-boreal krill species Thysanoessa inermis to variations in water temperature

T2 - coupling Hsp70 isoform expressions with metabolism

AU - Huenerlage, Kim

AU - Cascella, Kévin

AU - Corre, Erwan

AU - Toomey, Lola

AU - Lee, Chi Ying

AU - Buchholz, Friedrich

AU - Toullec, Jean Yves

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Recent studies have indicated a metabolic temperature sensitivity in both the arcto-boreal krill species Thysanoessa inermis and Thysanoessa raschii that may determine these species’ abundance and population persistence at lower latitudes (up to 40° N). T. inermis currently dominates the krill community in the Barents Sea and in the high Arctic Kongsfjord. We aimed to increase the knowledge on the upper thermal limit found in the latter species by estimating the CT50 value (19.7 °C) (critical temperature at which 50 % of animals are reactive) and by linking metabolic rate measurements with molecular approaches. Optical oxygen sensors were used to measure respiration rates in steps of 2 °C (from 0 to 16 °C). To follow the temperature-mediated mechanisms of passive response, i.e., as a proxy for molecular stress, molecular chaperone heat shock protein 70 (Hsp70) sequences were extracted from a transcriptome assembly, and the gene expression kinetics were monitored during an acute temperature exposure to 6 or 10 °C with subsequent recovery at 4 °C. Our results showed upregulation of hsp70 genes, especially the structurally constitutive and mitochondrial isoforms. These findings confirmed the temperature sensitivity of T. inermis and showed that the thermal stress took place before reaching the upper temperature limit estimated by respirometry at 12 °C. This study provides a baseline for further investigations into the thermal tolerances of arcto-boreal Thysanoessa spp. and comparisons with other krill species under different climatic regimes, especially Antarctica.

AB - Recent studies have indicated a metabolic temperature sensitivity in both the arcto-boreal krill species Thysanoessa inermis and Thysanoessa raschii that may determine these species’ abundance and population persistence at lower latitudes (up to 40° N). T. inermis currently dominates the krill community in the Barents Sea and in the high Arctic Kongsfjord. We aimed to increase the knowledge on the upper thermal limit found in the latter species by estimating the CT50 value (19.7 °C) (critical temperature at which 50 % of animals are reactive) and by linking metabolic rate measurements with molecular approaches. Optical oxygen sensors were used to measure respiration rates in steps of 2 °C (from 0 to 16 °C). To follow the temperature-mediated mechanisms of passive response, i.e., as a proxy for molecular stress, molecular chaperone heat shock protein 70 (Hsp70) sequences were extracted from a transcriptome assembly, and the gene expression kinetics were monitored during an acute temperature exposure to 6 or 10 °C with subsequent recovery at 4 °C. Our results showed upregulation of hsp70 genes, especially the structurally constitutive and mitochondrial isoforms. These findings confirmed the temperature sensitivity of T. inermis and showed that the thermal stress took place before reaching the upper temperature limit estimated by respirometry at 12 °C. This study provides a baseline for further investigations into the thermal tolerances of arcto-boreal Thysanoessa spp. and comparisons with other krill species under different climatic regimes, especially Antarctica.

UR - http://www.scopus.com/inward/record.url?scp=84983378953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983378953&partnerID=8YFLogxK

U2 - 10.1007/s12192-016-0720-6

DO - 10.1007/s12192-016-0720-6

M3 - Article

C2 - 27558691

AN - SCOPUS:84983378953

VL - 21

SP - 969

EP - 981

JO - Cell Stress and Chaperones

JF - Cell Stress and Chaperones

SN - 1355-8145

IS - 6

ER -