Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409

Chi Wen Lin, Dang Thuan Tran, Chi-Yung Lai, Yet Pole I, Chih Hung Wu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.

Original languageEnglish
Pages (from-to)1922-1929
Number of pages8
JournalBiomass and Bioenergy
Volume34
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Klebsiella oxytoca
Pennisetum
ethanol production
ethanol
Ethanol
yeast extract
Yeast
Fermentation
fermentation
yeast
lignocellulose
response surface methodology
powders
temperature
regression analysis
Regression analysis
microorganisms
Productivity
liquids
Powders

All Science Journal Classification (ASJC) codes

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

Cite this

Lin, Chi Wen ; Tran, Dang Thuan ; Lai, Chi-Yung ; I, Yet Pole ; Wu, Chih Hung. / Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409. In: Biomass and Bioenergy. 2010 ; Vol. 34, No. 12. pp. 1922-1929.
@article{03e60512283044b6aba6f5f17b493044,
title = "Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409",
abstract = "The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6{\%} confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.",
author = "Lin, {Chi Wen} and Tran, {Dang Thuan} and Chi-Yung Lai and I, {Yet Pole} and Wu, {Chih Hung}",
year = "2010",
month = "12",
day = "1",
doi = "10.1016/j.biombioe.2010.07.032",
language = "English",
volume = "34",
pages = "1922--1929",
journal = "Biomass and Bioenergy",
issn = "0961-9534",
publisher = "Elsevier Limited",
number = "12",

}

Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409. / Lin, Chi Wen; Tran, Dang Thuan; Lai, Chi-Yung; I, Yet Pole; Wu, Chih Hung.

In: Biomass and Bioenergy, Vol. 34, No. 12, 01.12.2010, p. 1922-1929.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409

AU - Lin, Chi Wen

AU - Tran, Dang Thuan

AU - Lai, Chi-Yung

AU - I, Yet Pole

AU - Wu, Chih Hung

PY - 2010/12/1

Y1 - 2010/12/1

N2 - The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.

AB - The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.

UR - http://www.scopus.com/inward/record.url?scp=78149241960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149241960&partnerID=8YFLogxK

U2 - 10.1016/j.biombioe.2010.07.032

DO - 10.1016/j.biombioe.2010.07.032

M3 - Article

VL - 34

SP - 1922

EP - 1929

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

SN - 0961-9534

IS - 12

ER -