Abstract
The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 1922-1929 |
| Number of pages | 8 |
| Journal | Biomass and Bioenergy |
| Volume | 34 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2010 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Forestry
- Renewable Energy, Sustainability and the Environment
- Agronomy and Crop Science
- Waste Management and Disposal
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Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409. / Lin, Chi Wen; Tran, Dang Thuan; Lai, Chi-Yung; I, Yet Pole; Wu, Chih Hung.
In: Biomass and Bioenergy, Vol. 34, No. 12, 01.12.2010, p. 1922-1929.Research output: Contribution to journal › Article
TY - JOUR
T1 - Response surface optimization for ethanol production from Pennisetum Alopecoider by Klebsiella oxytoca THLC0409
AU - Lin, Chi Wen
AU - Tran, Dang Thuan
AU - Lai, Chi-Yung
AU - I, Yet Pole
AU - Wu, Chih Hung
PY - 2010/12/1
Y1 - 2010/12/1
N2 - The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.
AB - The strain Klebsiella oxytoca THLC0409 was isolated from a lignocellulose-degrading microflora and used in the direct conversion of Napiergrass powder to ethanol. Central Composite Design (CCD), part of the Response Surface Methodology (RSM) was adopted to study the effects of the fermentation time, the initial pH of the liquid medium, the cultivation temperature, and the yeast extract (YE) concentration on ethanol production. A second-order model that relates the ethanol concentration to four basic factors was developed. Regression analysis revealed that the maximum ethanol concentration of 472 ppm could be reached at a fermentation time of about 11 days, an initial pH of 7.04, a cultivating temperature of 31 °C, and a YE concentration of 7.0 g l-1. The adjusted R-square of the regression model was 0.996, indicating a good fit at 99.6% confidence level. The highest ethanol yield and productivity were estimated to be 82 g kg-1 and 360 mg kg-1 h-1, respectively.
UR - http://www.scopus.com/inward/record.url?scp=78149241960&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78149241960&partnerID=8YFLogxK
U2 - 10.1016/j.biombioe.2010.07.032
DO - 10.1016/j.biombioe.2010.07.032
M3 - Article
AN - SCOPUS:78149241960
VL - 34
SP - 1922
EP - 1929
JO - Biomass and Bioenergy
JF - Biomass and Bioenergy
SN - 0961-9534
IS - 12
ER -