Relevance of phenolic diterpene constituents to antioxidant activity of supercritical CO2 extract from the leaves of rosemary

Chi Huang Chang, Charng Cherng Chyau, Chiu-Lan Hsieh, Yen Ying Wu, Yaw Bee Ker, Hau Yang Tsen, Robert Y. Peng

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Isolation of phenolic diterpene constituents from the freeze-dried leaves of Rosmarinus officinalis has been obtained by supercritical extraction with carbon dioxide. To determine the ideal conditions for the maximum yield of extract, nine different conditions using three levels of pressures (3000, 4000 and 5000 psi) in combination with three temperatures at 40, 60 and 80°C, respectively, in combination with the analyses of the corresponding antioxidant activities and constituents which existed in extracts has been investigated. The antioxidant activity of each obtained extract was determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals test. GC/MS method was used as an alternative to conventional HPLC method for the determination of the principal antioxidant constituents in extract, including phenolic diterpenes carnosic acid (CA) and carnosol (CAL). The confirmation of CA and CAL in extract was forward performed by subjecting HPLC isolates from extract into an ion trap mass spectrometer through an electrospray ionization (ESI) interface for MS/MS analysis. These results indicate that an ideal extraction process was obtained at 5000 psi and 80°C with an extraction yield of 4.27% (w/w) and rich in phenolic antioxidants CA and CAL as contents of 35.23 and 0.46 mg g-1 in extract, respectively.

Original languageEnglish
Pages (from-to)76-90
Number of pages15
JournalNatural Product Research
Volume22
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

Diterpenes
rosemary
diterpenoids
Antioxidants
antioxidant activity
carbon dioxide
extracts
leaves
Rosmarinus
High Pressure Liquid Chromatography
Electrospray ionization
Mass spectrometers
acids
Carbon Dioxide
antioxidants
Rosmarinus officinalis
Ions
Pressure
Temperature
spectrometers

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

Cite this

Chang, Chi Huang ; Chyau, Charng Cherng ; Hsieh, Chiu-Lan ; Wu, Yen Ying ; Ker, Yaw Bee ; Tsen, Hau Yang ; Peng, Robert Y. / Relevance of phenolic diterpene constituents to antioxidant activity of supercritical CO2 extract from the leaves of rosemary. In: Natural Product Research. 2008 ; Vol. 22, No. 1. pp. 76-90.
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Relevance of phenolic diterpene constituents to antioxidant activity of supercritical CO2 extract from the leaves of rosemary. / Chang, Chi Huang; Chyau, Charng Cherng; Hsieh, Chiu-Lan; Wu, Yen Ying; Ker, Yaw Bee; Tsen, Hau Yang; Peng, Robert Y.

In: Natural Product Research, Vol. 22, No. 1, 01.01.2008, p. 76-90.

Research output: Contribution to journalArticle

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