Reducing the expression of glutathione transferase D mRNA in Drosophila melanogaster exposed to phenol and aniline

K. Ding, Y. Chien, C. Chien

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Phenol and aniline are toxic to animals. The purpose of the present study was to examine the expression of glutathione transferase D mRNA in fruit flies altered by long-term exposure to phenol and aniline. Changes in the amount of mRNA were measured by a semiquantitative reverse-transcription polymerase chain reaction assay. The level of each glutathione transferase D mRNA expressed in the phenol-treated and aniline-treated strains of adult fruit flies differed after chemical treatment. Aniline was more potent than phenol in suppressing the expression of cytosolic glutathione transferase D mRNA. Aniline reduced the level of glutathione transferase mRNA expressed in the aniline-treated strain to less than a 0.5 fraction as compared to that measured in the wild-type strain. But phenol was only able to suppress the GstD7 and GstD4 mRNAs expressed in the phenol-treated strain. Neither aniline nor phenol reduced the expression of microsomal glutathione transferase mRNA in fruit flies.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalEnvironmental Toxicology
Issue number5
Publication statusPublished - 2005 Oct 17


All Science Journal Classification (ASJC) codes

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

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