Reducing the expression of glutathione transferase D mRNA in Drosophila melanogaster exposed to phenol and aniline

K. Ding, Y. Chien, C. Chien

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Phenol and aniline are toxic to animals. The purpose of the present study was to examine the expression of glutathione transferase D mRNA in fruit flies altered by long-term exposure to phenol and aniline. Changes in the amount of mRNA were measured by a semiquantitative reverse-transcription polymerase chain reaction assay. The level of each glutathione transferase D mRNA expressed in the phenol-treated and aniline-treated strains of adult fruit flies differed after chemical treatment. Aniline was more potent than phenol in suppressing the expression of cytosolic glutathione transferase D mRNA. Aniline reduced the level of glutathione transferase mRNA expressed in the aniline-treated strain to less than a 0.5 fraction as compared to that measured in the wild-type strain. But phenol was only able to suppress the GstD7 and GstD4 mRNAs expressed in the phenol-treated strain. Neither aniline nor phenol reduced the expression of microsomal glutathione transferase mRNA in fruit flies.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalEnvironmental Toxicology
Volume20
Issue number5
DOIs
Publication statusPublished - 2005 Oct 17

Fingerprint

Aniline
Phenol
Glutathione Transferase
Drosophila melanogaster
Phenols
phenol
Messenger RNA
Fruits
Diptera
Fruit
fruit
Poisons
Polymerase chain reaction
Transcription
aniline
Glutathione
polymerase chain reaction
Reverse Transcription
Assays
Animals

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

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abstract = "Phenol and aniline are toxic to animals. The purpose of the present study was to examine the expression of glutathione transferase D mRNA in fruit flies altered by long-term exposure to phenol and aniline. Changes in the amount of mRNA were measured by a semiquantitative reverse-transcription polymerase chain reaction assay. The level of each glutathione transferase D mRNA expressed in the phenol-treated and aniline-treated strains of adult fruit flies differed after chemical treatment. Aniline was more potent than phenol in suppressing the expression of cytosolic glutathione transferase D mRNA. Aniline reduced the level of glutathione transferase mRNA expressed in the aniline-treated strain to less than a 0.5 fraction as compared to that measured in the wild-type strain. But phenol was only able to suppress the GstD7 and GstD4 mRNAs expressed in the phenol-treated strain. Neither aniline nor phenol reduced the expression of microsomal glutathione transferase mRNA in fruit flies.",
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Reducing the expression of glutathione transferase D mRNA in Drosophila melanogaster exposed to phenol and aniline. / Ding, K.; Chien, Y.; Chien, C.

In: Environmental Toxicology, Vol. 20, No. 5, 17.10.2005, p. 507-512.

Research output: Contribution to journalArticle

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