Real time monitoring of superoxide generation in zebrafish liver using a sensitive fiber-optic probe

Yu Chung Chang, Che Wei Hsu, Hsu Fu Chang, Chuian Fu Ken, Ya Ging Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The genetic feature of zebrafish is similar to that of mammal, which renders zebrafish an emerging animal model for biotechnology. A recently discovered circularly permuted yellow fluorescent protein (cpYFP) is a superoxide-specific indicator. We specifically transfect the cpYFP into the liver of zebrafish and use a fiber-optic fluorescent probe to conduct noninvasive monitoring of superoxide concentrations in the fish liver in vivo in real time. Several superoxide-generating and scavenging reagents are tested. The time courses of superoxide concentration variations are presented. This technique can be used as a high throughput pharmaceutical test platform.

Original languageEnglish
Title of host publication2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013
Pages200-201
Number of pages2
DOIs
Publication statusPublished - 2013 Nov 29
Event2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013 - Hsinchu, Taiwan
Duration: 2013 Jul 62013 Jul 9

Publication series

Name2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013

Other

Other2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013
CountryTaiwan
CityHsinchu
Period13-07-0613-07-09

Fingerprint

Liver
Fiber optics
Proteins
Mammals
Monitoring
Scavenging
Biotechnology
Drug products
Fish
Animals
Throughput

All Science Journal Classification (ASJC) codes

  • Information Systems

Cite this

Chang, Y. C., Hsu, C. W., Chang, H. F., Ken, C. F., & Liu, Y. G. (2013). Real time monitoring of superoxide generation in zebrafish liver using a sensitive fiber-optic probe. In 2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013 (pp. 200-201). [6621550] (2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013). https://doi.org/10.1109/ICAIT.2013.6621550
Chang, Yu Chung ; Hsu, Che Wei ; Chang, Hsu Fu ; Ken, Chuian Fu ; Liu, Ya Ging. / Real time monitoring of superoxide generation in zebrafish liver using a sensitive fiber-optic probe. 2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013. 2013. pp. 200-201 (2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013).
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abstract = "The genetic feature of zebrafish is similar to that of mammal, which renders zebrafish an emerging animal model for biotechnology. A recently discovered circularly permuted yellow fluorescent protein (cpYFP) is a superoxide-specific indicator. We specifically transfect the cpYFP into the liver of zebrafish and use a fiber-optic fluorescent probe to conduct noninvasive monitoring of superoxide concentrations in the fish liver in vivo in real time. Several superoxide-generating and scavenging reagents are tested. The time courses of superoxide concentration variations are presented. This technique can be used as a high throughput pharmaceutical test platform.",
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Chang, YC, Hsu, CW, Chang, HF, Ken, CF & Liu, YG 2013, Real time monitoring of superoxide generation in zebrafish liver using a sensitive fiber-optic probe. in 2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013., 6621550, 2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013, pp. 200-201, 2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013, Hsinchu, Taiwan, 13-07-06. https://doi.org/10.1109/ICAIT.2013.6621550

Real time monitoring of superoxide generation in zebrafish liver using a sensitive fiber-optic probe. / Chang, Yu Chung; Hsu, Che Wei; Chang, Hsu Fu; Ken, Chuian Fu; Liu, Ya Ging.

2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013. 2013. p. 200-201 6621550 (2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chang YC, Hsu CW, Chang HF, Ken CF, Liu YG. Real time monitoring of superoxide generation in zebrafish liver using a sensitive fiber-optic probe. In 2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013. 2013. p. 200-201. 6621550. (2013 IEEE 6th International Conference on Advanced Infocomm Technology, ICAIT 2013). https://doi.org/10.1109/ICAIT.2013.6621550