Abstract
Superoxide anion is the primary oxygen free radical generated in mitochondria that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express fluorescent proteins, which are recently developed as reversible superoxide-specific indicators, in the liver. A fiber-optic fluorescent probe was used to noninvasively monitor superoxide generation in the liver in real time. The fish were placed in microfluidic channels for manipulation and reagents administration. Several superoxide-inducing and scavenging reagents were administrated onto the fish to investigate their effects on superoxide anion balancing. The biochemical dynamics of superoxide due to the application reagents were revealed in the transient behaviors of fluorescence time courses. With the ability to monitor superoxide dynamics in vivo in real time, this method can be used as an in vivo pharmaceutical screening platform.
| Original language | English |
|---|---|
| Title of host publication | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII |
| Publisher | SPIE |
| ISBN (Print) | 9780819498601 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
| Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII - San Francisco, CA, United States Duration: 2014 Feb 3 → 2014 Feb 6 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 8947 |
| ISSN (Print) | 1605-7422 |
Other
| Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII |
|---|---|
| Country | United States |
| City | San Francisco, CA |
| Period | 14-02-03 → 14-02-06 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
Cite this
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Real time monitoring of superoxide dynamics in vivo through fluorescent proteins using a sensitive fiber probe. / Chang, Yu Chung; Ken, Chuian Fu; Hsu, Che Wei; Liu, Ya Ging.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII. SPIE, 2014. 894721 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8947).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Real time monitoring of superoxide dynamics in vivo through fluorescent proteins using a sensitive fiber probe
AU - Chang, Yu Chung
AU - Ken, Chuian Fu
AU - Hsu, Che Wei
AU - Liu, Ya Ging
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Superoxide anion is the primary oxygen free radical generated in mitochondria that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express fluorescent proteins, which are recently developed as reversible superoxide-specific indicators, in the liver. A fiber-optic fluorescent probe was used to noninvasively monitor superoxide generation in the liver in real time. The fish were placed in microfluidic channels for manipulation and reagents administration. Several superoxide-inducing and scavenging reagents were administrated onto the fish to investigate their effects on superoxide anion balancing. The biochemical dynamics of superoxide due to the application reagents were revealed in the transient behaviors of fluorescence time courses. With the ability to monitor superoxide dynamics in vivo in real time, this method can be used as an in vivo pharmaceutical screening platform.
AB - Superoxide anion is the primary oxygen free radical generated in mitochondria that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express fluorescent proteins, which are recently developed as reversible superoxide-specific indicators, in the liver. A fiber-optic fluorescent probe was used to noninvasively monitor superoxide generation in the liver in real time. The fish were placed in microfluidic channels for manipulation and reagents administration. Several superoxide-inducing and scavenging reagents were administrated onto the fish to investigate their effects on superoxide anion balancing. The biochemical dynamics of superoxide due to the application reagents were revealed in the transient behaviors of fluorescence time courses. With the ability to monitor superoxide dynamics in vivo in real time, this method can be used as an in vivo pharmaceutical screening platform.
UR - http://www.scopus.com/inward/record.url?scp=84901802580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901802580&partnerID=8YFLogxK
U2 - 10.1117/12.2038218
DO - 10.1117/12.2038218
M3 - Conference contribution
AN - SCOPUS:84901802580
SN - 9780819498601
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
PB - SPIE
ER -