Abstract
Superoxide anion is the key radical that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express yellow fluorescent proteins, a reversible superoxide-specific indicator, in the liver and used a fiber-optic fluorescent probe to noninvasively monitor the superoxide concentration in real time. Several superoxide-inducing and scavenging reagents were administrated onto the fish to alter superoxide concentrations. The distinct biochemical pathways of the reagents can be discerned from the transient behaviors of fluorescence time courses. These results demonstrate the feasibility of this method for analyzing superoxide dynamics and its potential as an in vivo pharmaceutical screening platform.
| Original language | English |
|---|---|
| Pages (from-to) | 1702-1709 |
| Number of pages | 8 |
| Journal | Biomedical Optics Express |
| Volume | 4 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2013 Sep 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Atomic and Molecular Physics, and Optics
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Real time in vivo investigation of superoxide dynamics in zebrafish liver using a single-fiber fluorescent probe. / Chang, Yu Chung; Ken, Chuian Fu; Hsu, Che Wei; Liu, Ya Ging.
In: Biomedical Optics Express, Vol. 4, No. 9, 01.09.2013, p. 1702-1709.Research output: Contribution to journal › Article
TY - JOUR
T1 - Real time in vivo investigation of superoxide dynamics in zebrafish liver using a single-fiber fluorescent probe
AU - Chang, Yu Chung
AU - Ken, Chuian Fu
AU - Hsu, Che Wei
AU - Liu, Ya Ging
PY - 2013/9/1
Y1 - 2013/9/1
N2 - Superoxide anion is the key radical that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express yellow fluorescent proteins, a reversible superoxide-specific indicator, in the liver and used a fiber-optic fluorescent probe to noninvasively monitor the superoxide concentration in real time. Several superoxide-inducing and scavenging reagents were administrated onto the fish to alter superoxide concentrations. The distinct biochemical pathways of the reagents can be discerned from the transient behaviors of fluorescence time courses. These results demonstrate the feasibility of this method for analyzing superoxide dynamics and its potential as an in vivo pharmaceutical screening platform.
AB - Superoxide anion is the key radical that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express yellow fluorescent proteins, a reversible superoxide-specific indicator, in the liver and used a fiber-optic fluorescent probe to noninvasively monitor the superoxide concentration in real time. Several superoxide-inducing and scavenging reagents were administrated onto the fish to alter superoxide concentrations. The distinct biochemical pathways of the reagents can be discerned from the transient behaviors of fluorescence time courses. These results demonstrate the feasibility of this method for analyzing superoxide dynamics and its potential as an in vivo pharmaceutical screening platform.
UR - http://www.scopus.com/inward/record.url?scp=84883394135&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883394135&partnerID=8YFLogxK
U2 - 10.1364/BOE.4.001702
DO - 10.1364/BOE.4.001702
M3 - Article
C2 - 24129412
AN - SCOPUS:84883394135
VL - 4
SP - 1702
EP - 1709
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 9
ER -