Real time in vivo investigation of superoxide dynamics in zebrafish liver using a single-fiber fluorescent probe

Yu Chung Chang, Chuian Fu Ken, Che Wei Hsu, Ya Ging Liu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Superoxide anion is the key radical that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express yellow fluorescent proteins, a reversible superoxide-specific indicator, in the liver and used a fiber-optic fluorescent probe to noninvasively monitor the superoxide concentration in real time. Several superoxide-inducing and scavenging reagents were administrated onto the fish to alter superoxide concentrations. The distinct biochemical pathways of the reagents can be discerned from the transient behaviors of fluorescence time courses. These results demonstrate the feasibility of this method for analyzing superoxide dynamics and its potential as an in vivo pharmaceutical screening platform.

Original languageEnglish
Pages (from-to)1702-1709
Number of pages8
JournalBiomedical Optics Express
Volume4
Issue number9
DOIs
Publication statusPublished - 2013 Sep 1

Fingerprint

inorganic peroxides
Zebrafish
Fluorescent Dyes
liver
Superoxides
fibers
probes
Liver
reagents
scavenging
fishes
fiber optics
Fishes
Oxidative Stress
screening
platforms
Fluorescence
anions
proteins
fluorescence

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

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abstract = "Superoxide anion is the key radical that causes intracellular oxidative stress. The lack of a method to directly monitor superoxide concentration in vivo in real time has severely hindered our understanding on its pathophysiology. We made transgenic zebrafish to specifically express yellow fluorescent proteins, a reversible superoxide-specific indicator, in the liver and used a fiber-optic fluorescent probe to noninvasively monitor the superoxide concentration in real time. Several superoxide-inducing and scavenging reagents were administrated onto the fish to alter superoxide concentrations. The distinct biochemical pathways of the reagents can be discerned from the transient behaviors of fluorescence time courses. These results demonstrate the feasibility of this method for analyzing superoxide dynamics and its potential as an in vivo pharmaceutical screening platform.",
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Real time in vivo investigation of superoxide dynamics in zebrafish liver using a single-fiber fluorescent probe. / Chang, Yu Chung; Ken, Chuian Fu; Hsu, Che Wei; Liu, Ya Ging.

In: Biomedical Optics Express, Vol. 4, No. 9, 01.09.2013, p. 1702-1709.

Research output: Contribution to journalArticle

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