Quantification of dendrimer nanoparticle targeting on tumor cells by two-photon excitation fluorescence through a dual-clad optical fiber

Yu-Chung Chang, Thommey P. Thomas, Jing Yong Ye, Alina Kotlyar, Zhengyi Cao, Istvan J. Majoros, James R. Baker, Theodore B. Norris

Research output: Contribution to conferencePaper

Abstract

We report the use of a dual-clad optical fiber to conduct quantitative two-photon excited fluorescence measurements in vitro and in vivo inside tumors. The ability to detect nanomolar concentrations of tumor cell targeting nanoparticles with this dual-clad fiber scheme is confirmed. The results indicate that this technique is an appropriate tool to quantify low concentrations of targeted fluorescence probes in vivo in deep tissue.

Original languageEnglish
Publication statusPublished - 2008 Dec 1
EventBiomedical Optics, BIOMED 2008 - St. Petersburg, FL, United States
Duration: 2008 Mar 162008 Mar 19

Other

OtherBiomedical Optics, BIOMED 2008
CountryUnited States
CitySt. Petersburg, FL
Period08-03-1608-03-19

Fingerprint

Dendrimers
dendrimers
Optical fibers
Tumors
tumors
Photons
optical fibers
Fluorescence
Cells
Nanoparticles
fluorescence
nanoparticles
photons
excitation
low concentrations
Tissue
fibers
Fibers
probes

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Chang, Y-C., Thomas, T. P., Ye, J. Y., Kotlyar, A., Cao, Z., Majoros, I. J., ... Norris, T. B. (2008). Quantification of dendrimer nanoparticle targeting on tumor cells by two-photon excitation fluorescence through a dual-clad optical fiber. Paper presented at Biomedical Optics, BIOMED 2008, St. Petersburg, FL, United States.
Chang, Yu-Chung ; Thomas, Thommey P. ; Ye, Jing Yong ; Kotlyar, Alina ; Cao, Zhengyi ; Majoros, Istvan J. ; Baker, James R. ; Norris, Theodore B. / Quantification of dendrimer nanoparticle targeting on tumor cells by two-photon excitation fluorescence through a dual-clad optical fiber. Paper presented at Biomedical Optics, BIOMED 2008, St. Petersburg, FL, United States.
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abstract = "We report the use of a dual-clad optical fiber to conduct quantitative two-photon excited fluorescence measurements in vitro and in vivo inside tumors. The ability to detect nanomolar concentrations of tumor cell targeting nanoparticles with this dual-clad fiber scheme is confirmed. The results indicate that this technique is an appropriate tool to quantify low concentrations of targeted fluorescence probes in vivo in deep tissue.",
author = "Yu-Chung Chang and Thomas, {Thommey P.} and Ye, {Jing Yong} and Alina Kotlyar and Zhengyi Cao and Majoros, {Istvan J.} and Baker, {James R.} and Norris, {Theodore B.}",
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Chang, Y-C, Thomas, TP, Ye, JY, Kotlyar, A, Cao, Z, Majoros, IJ, Baker, JR & Norris, TB 2008, 'Quantification of dendrimer nanoparticle targeting on tumor cells by two-photon excitation fluorescence through a dual-clad optical fiber' Paper presented at Biomedical Optics, BIOMED 2008, St. Petersburg, FL, United States, 08-03-16 - 08-03-19, .

Quantification of dendrimer nanoparticle targeting on tumor cells by two-photon excitation fluorescence through a dual-clad optical fiber. / Chang, Yu-Chung; Thomas, Thommey P.; Ye, Jing Yong; Kotlyar, Alina; Cao, Zhengyi; Majoros, Istvan J.; Baker, James R.; Norris, Theodore B.

2008. Paper presented at Biomedical Optics, BIOMED 2008, St. Petersburg, FL, United States.

Research output: Contribution to conferencePaper

TY - CONF

T1 - Quantification of dendrimer nanoparticle targeting on tumor cells by two-photon excitation fluorescence through a dual-clad optical fiber

AU - Chang, Yu-Chung

AU - Thomas, Thommey P.

AU - Ye, Jing Yong

AU - Kotlyar, Alina

AU - Cao, Zhengyi

AU - Majoros, Istvan J.

AU - Baker, James R.

AU - Norris, Theodore B.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - We report the use of a dual-clad optical fiber to conduct quantitative two-photon excited fluorescence measurements in vitro and in vivo inside tumors. The ability to detect nanomolar concentrations of tumor cell targeting nanoparticles with this dual-clad fiber scheme is confirmed. The results indicate that this technique is an appropriate tool to quantify low concentrations of targeted fluorescence probes in vivo in deep tissue.

AB - We report the use of a dual-clad optical fiber to conduct quantitative two-photon excited fluorescence measurements in vitro and in vivo inside tumors. The ability to detect nanomolar concentrations of tumor cell targeting nanoparticles with this dual-clad fiber scheme is confirmed. The results indicate that this technique is an appropriate tool to quantify low concentrations of targeted fluorescence probes in vivo in deep tissue.

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M3 - Paper

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Chang Y-C, Thomas TP, Ye JY, Kotlyar A, Cao Z, Majoros IJ et al. Quantification of dendrimer nanoparticle targeting on tumor cells by two-photon excitation fluorescence through a dual-clad optical fiber. 2008. Paper presented at Biomedical Optics, BIOMED 2008, St. Petersburg, FL, United States.