Quantification of Captopril in Urine Through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Using 4-Mercaptobenzoic Acid-Capped Gold Nanoparticles as an Internal Standard

Wen Tsen Chen, Cheng Kang Chiang, Yang-Wei Lin, Huan Tsung Chang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We have developed a new internal standard method for the determination of the concentration of captopril (CAP) through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using gold nanoparticles (Au NPs). This approach provided linearity for CAP over the concentration range 2.525 M (R2 = 0.987), with a limit of detection (signal-to-noise ratio = 3) of 1.0 M. The spot-to-spot variations in the concentration of CAP through SALDI-MS analyses performed in the absence and presence of the internal standard were 26% and 9%, respectively (15 measurements). This approach provides simplicity, accuracy, precision, and great reproducibility to the determination of the levels of CAP in human urine samples.

Original languageEnglish
Pages (from-to)864-867
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Volume21
Issue number5
DOIs
Publication statusPublished - 2010 May 1

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Captopril
Gold
Nanoparticles
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Urine
Signal-To-Noise Ratio
Limit of Detection
Signal to noise ratio
4-mercaptobenzoate

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Spectroscopy

Cite this

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abstract = "We have developed a new internal standard method for the determination of the concentration of captopril (CAP) through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using gold nanoparticles (Au NPs). This approach provided linearity for CAP over the concentration range 2.525 M (R2 = 0.987), with a limit of detection (signal-to-noise ratio = 3) of 1.0 M. The spot-to-spot variations in the concentration of CAP through SALDI-MS analyses performed in the absence and presence of the internal standard were 26{\%} and 9{\%}, respectively (15 measurements). This approach provides simplicity, accuracy, precision, and great reproducibility to the determination of the levels of CAP in human urine samples.",
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AU - Chiang, Cheng Kang

AU - Lin, Yang-Wei

AU - Chang, Huan Tsung

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