We have developed a new internal standard method for the determination of the concentration of captopril (CAP) through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using gold nanoparticles (Au NPs). This approach provided linearity for CAP over the concentration range 2.525 M (R2 = 0.987), with a limit of detection (signal-to-noise ratio = 3) of 1.0 M. The spot-to-spot variations in the concentration of CAP through SALDI-MS analyses performed in the absence and presence of the internal standard were 26% and 9%, respectively (15 measurements). This approach provides simplicity, accuracy, precision, and great reproducibility to the determination of the levels of CAP in human urine samples.
|Number of pages||4|
|Journal||Journal of the American Society for Mass Spectrometry|
|Publication status||Published - 2010 May 1|
All Science Journal Classification (ASJC) codes
- Structural Biology