Putative phospholipid hydroperoxide glutathione peroxidase from Antrodia camphorata

Hsueh T. Chen, Choa Y. Lin, Chuian-Fu Ken, Lisa Wen, Chi Tsai Lin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Glutathione Peroxidases (GPxs) play important roles in antioxidation. A cDNA (Ac-PHGPx, 764 bp) encoding a putative phospholipid hydroperoxide glutathione peroxidase (PHGPx) from Antrodia camphorata has been cloned. The deduced amino acid sequence is conserved among the reported GPxs. To characterize the Ac-PHGPx, the coding region was subcloned into pYEX-S1 and transformed into Saccharomyces cerevisiae. The recombinant 6His-tagged Ac-PHGPx was expressed and purified by Ni2+-nitrilotriacetic acid Sepharose. The purified enzyme showed a predominant band with molecular mass of ∼18 kDa on 12% SDS-PAGE. The enzyme retained 50% activity at 60 °C for 8 min. The enzyme was most active at pH 9. The enzyme showed 42% activity after incubation with trypsin at 37 °C for 40 min. In addition, the ability of Ac-PHGPx to protect intact supercoiled plasmid DNA from {radical dot}OH induced nicking was demonstrated.

Original languageEnglish
Pages (from-to)476-482
Number of pages7
JournalFood Chemistry
Volume115
Issue number2
DOIs
Publication statusPublished - 2009 Jul 15

Fingerprint

phospholipid-hydroperoxide glutathione peroxidase
Antrodia
Taiwanofungus camphoratus
Enzymes
Glutathione Peroxidase
enzymes
glutathione peroxidase
nitrilotriacetic acid
Nitrilotriacetic Acid
Superhelical DNA
Molecular mass
Yeast
Sepharose
Trypsin
trypsin
agarose
Saccharomyces cerevisiae
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
plasmids

All Science Journal Classification (ASJC) codes

  • Food Science
  • Analytical Chemistry

Cite this

Chen, Hsueh T. ; Lin, Choa Y. ; Ken, Chuian-Fu ; Wen, Lisa ; Lin, Chi Tsai. / Putative phospholipid hydroperoxide glutathione peroxidase from Antrodia camphorata. In: Food Chemistry. 2009 ; Vol. 115, No. 2. pp. 476-482.
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Putative phospholipid hydroperoxide glutathione peroxidase from Antrodia camphorata. / Chen, Hsueh T.; Lin, Choa Y.; Ken, Chuian-Fu; Wen, Lisa; Lin, Chi Tsai.

In: Food Chemistry, Vol. 115, No. 2, 15.07.2009, p. 476-482.

Research output: Contribution to journalArticle

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