Protective effect of extracts of Mesona procumbens Hemsl. on DNA damage in human lymphocytes exposed to hydrogen peroxide and UV irradiation

G. C. Yen, Y. L. Hung, Chiu-Lan Hsieh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In the present study, the protective effect of water extracts from Hsian-tsao (WEHT) on DNA damage in human lymphocytes induced by UV-C and/or H2O2 was evaluated using single-cell electrophoresis (comet assay). No toxicity was found in WEHT towards human lymphocytes. WEHT did not cause DNA damage at lower concentrations of 0.05 and 0.1 mg/ml, while it did cause slight DNA damage at a concentration of 0.5-2.5 mg/ml when compared with the control group. When WEHT was mixed with H2O2 for reaction, it exhibited a slight inhibitory effect on DNA damage induced by H2O2. Moreover, when WEHT and lymphocytes were irradiated by UV-C and then incubated for 35 min, the DNA damage decreased with an increase of the concentration of WEHT. Thus, WEHT could reduce UV-C-induced DNA damage, and WEHT had a more protective effect on UV-C than on H2O2-induced DNA damage. The protective effect of WEHT on DNA damage might be due to the fact that it contains polyphenol compounds and/or other active components. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)747-754
Number of pages8
JournalFood and Chemical Toxicology
Volume38
Issue number9
DOIs
Publication statusPublished - 2000 Sep 1

All Science Journal Classification (ASJC) codes

  • Food Science
  • Toxicology

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