Production of active nonglycosylated recombinant B-chain of type-2 ribosome-inactivating protein from viscum articulatum and its biological effects on peripheral blood mononuclear cells

Ching Hsiang Hsu, Tzu Li Lu, Jing Yuan Chuang, Jai Sing Yang, Shau Ting Chiu, Nai Wan Hsiao, Mei Chen Wu, Shih Hsiung Wu

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Abstract

Type-2 ribosome-inactivating proteins, composed of a toxic A-chain and lectin-like B-chain, display various biological functions, including cytotoxicity and immunomodulation. We here cloned the lectin-like B-chain encoding fragment of a newly identified type-2 RIP gene, articulatin gene, from Viscum articulatum, into a bacterial expression vector to obtain nonglycosylated recombinant protein expressed in inclusion bodies. After purification and protein refolding, soluble refolded recombinant articulatin B-chain (rATB) showed lectin activity specific toward galactoside moiety and was stably maintained while stored in low ionic strength solution. Despite lacking glycosylation, rATB actively bound leukocytes with preferential binding to monocytes and in vitro stimulated PBMCs to release cytokines without obvious cytotoxicity. These results implicated such a B-chain fragment as a potential immunomodulator.

Original languageEnglish
Article number283747
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
Publication statusPublished - 2011 Jun 22

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All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

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