of the aphid Schlechtendalia chinensis

C. ‐Y Lai, P. Baumann, N. A. Moran

Research output: Contribution to journalReview article

24 Citations (Scopus)

Abstract

Two DNA fragments (3941 and 7152 base pairs) from the procaryotic endosymbiont Buchnera of the aphid Schlechtendalia chinensis were cloned and sequenced. The smaller fragment contained trpEG and the larger fragment contained trpDC(F)BA, genes coding for enzymes of the tryptophan biosynthetic pathway which convert chorismate to tryptophan. Both of these gene clusters were present as one copy on the endosymbiont chromosome and probably constitute two transcription units. The deduced amino acid sequences of the proteins was 51–61% identical to the corresponding proteins were previously studied in Buchnera of the aphid Schizaphis graminum. In this endosymbiont, trpEG is amplified and located on a plasmid, whereas, in the endosymbiont of S. chinensis, as in other eubacteria, trpEG occurs as a single copy on the bacterial chromosome. Implications of these findings for the evolution of this mutualistic association are discussed.

Original languageEnglish
Pages (from-to)47-59
Number of pages13
JournalInsect Molecular Biology
Volume4
Issue number1
DOIs
Publication statusPublished - 1995 Feb

Fingerprint

Aphids
Buchnera
endosymbionts
Aphidoidea
Tryptophan
Bacterial Chromosomes
tryptophan
Biosynthetic Pathways
Multigene Family
chromosomes
Schizaphis graminum
Eubacteria
Base Pairing
Amino Acid Sequence
Proteins
Plasmids
Chromosomes
multigene family
Bacteria
biochemical pathways

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

Lai, C. ‐Y ; Baumann, P. ; Moran, N. A. / of the aphid Schlechtendalia chinensis. In: Insect Molecular Biology. 1995 ; Vol. 4, No. 1. pp. 47-59.
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of the aphid Schlechtendalia chinensis. / Lai, C. ‐Y; Baumann, P.; Moran, N. A.

In: Insect Molecular Biology, Vol. 4, No. 1, 02.1995, p. 47-59.

Research output: Contribution to journalReview article

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AU - Lai, C. ‐Y

AU - Baumann, P.

AU - Moran, N. A.

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AB - Two DNA fragments (3941 and 7152 base pairs) from the procaryotic endosymbiont Buchnera of the aphid Schlechtendalia chinensis were cloned and sequenced. The smaller fragment contained trpEG and the larger fragment contained trpDC(F)BA, genes coding for enzymes of the tryptophan biosynthetic pathway which convert chorismate to tryptophan. Both of these gene clusters were present as one copy on the endosymbiont chromosome and probably constitute two transcription units. The deduced amino acid sequences of the proteins was 51–61% identical to the corresponding proteins were previously studied in Buchnera of the aphid Schizaphis graminum. In this endosymbiont, trpEG is amplified and located on a plasmid, whereas, in the endosymbiont of S. chinensis, as in other eubacteria, trpEG occurs as a single copy on the bacterial chromosome. Implications of these findings for the evolution of this mutualistic association are discussed.

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