Abstract
The presence, characteristics and localization of nitric oxide synthase (NOS) in the eyestalk ganglia of the crayfish (Procambarus clarkii) were investigated. Biochemical assay indicated that NOS activity was significantly inhibited by N(G)-nitro-L-arginine (a NOS inhibitor), fluphenazine-N-2- chloroethane (a calmodulin inhibitor) as well as by omitting β-NADPH or Ca2+ from the incubations. Immunohistochemical studies localized NOS immunoreactivity (IR) to the first chiasma and the sinus gland. Finally, a NOS-immunoreactive protein (138 kDa) was detected by Western blot analysis. These data demonstrate for the first time the existence in crustacean eyestalk ganglia of NOS with biochemical characteristics similar to mammalian constitutive NOS. The localization of NOS-IR suggests that NO may be involved in regulating visual processes and neuroendocrine function in crustaceans. (C) 2000 Lippincott Williams and Wilkins.
| Original language | English |
|---|---|
| Pages (from-to) | 1273-1276 |
| Number of pages | 4 |
| Journal | NeuroReport |
| Volume | 11 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2000 Apr 27 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
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Nitric oxide synthase activity and immunoreactivity in the crayfish Procambarus clarkii. / Lee, Chi Ying; Zou, Hong Shin; Yau, Shiou Mei; Ju, Yuh Rur; Liau, Ching Sheyen.
In: NeuroReport, Vol. 11, No. 6, 27.04.2000, p. 1273-1276.Research output: Contribution to journal › Article
TY - JOUR
T1 - Nitric oxide synthase activity and immunoreactivity in the crayfish Procambarus clarkii
AU - Lee, Chi Ying
AU - Zou, Hong Shin
AU - Yau, Shiou Mei
AU - Ju, Yuh Rur
AU - Liau, Ching Sheyen
PY - 2000/4/27
Y1 - 2000/4/27
N2 - The presence, characteristics and localization of nitric oxide synthase (NOS) in the eyestalk ganglia of the crayfish (Procambarus clarkii) were investigated. Biochemical assay indicated that NOS activity was significantly inhibited by N(G)-nitro-L-arginine (a NOS inhibitor), fluphenazine-N-2- chloroethane (a calmodulin inhibitor) as well as by omitting β-NADPH or Ca2+ from the incubations. Immunohistochemical studies localized NOS immunoreactivity (IR) to the first chiasma and the sinus gland. Finally, a NOS-immunoreactive protein (138 kDa) was detected by Western blot analysis. These data demonstrate for the first time the existence in crustacean eyestalk ganglia of NOS with biochemical characteristics similar to mammalian constitutive NOS. The localization of NOS-IR suggests that NO may be involved in regulating visual processes and neuroendocrine function in crustaceans. (C) 2000 Lippincott Williams and Wilkins.
AB - The presence, characteristics and localization of nitric oxide synthase (NOS) in the eyestalk ganglia of the crayfish (Procambarus clarkii) were investigated. Biochemical assay indicated that NOS activity was significantly inhibited by N(G)-nitro-L-arginine (a NOS inhibitor), fluphenazine-N-2- chloroethane (a calmodulin inhibitor) as well as by omitting β-NADPH or Ca2+ from the incubations. Immunohistochemical studies localized NOS immunoreactivity (IR) to the first chiasma and the sinus gland. Finally, a NOS-immunoreactive protein (138 kDa) was detected by Western blot analysis. These data demonstrate for the first time the existence in crustacean eyestalk ganglia of NOS with biochemical characteristics similar to mammalian constitutive NOS. The localization of NOS-IR suggests that NO may be involved in regulating visual processes and neuroendocrine function in crustaceans. (C) 2000 Lippincott Williams and Wilkins.
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U2 - 10.1097/00001756-200004270-00026
DO - 10.1097/00001756-200004270-00026
M3 - Article
C2 - 10817606
AN - SCOPUS:0034720176
VL - 11
SP - 1273
EP - 1276
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 6
ER -