Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins

Cheng Kang Chiang; Ni Chen Chiang; Zong Hong Lin; Guo Yu Lan; Yang Wei Lin; Huan Tsung Chang

doi:10.1016/j.jasms.2010.02.028

Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins

Cheng Kang Chiang, Ni Chen Chiang, Zong Hong Lin, Guo Yu Lan, Yang Wei Lin, Huan Tsung Chang

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe₃O₄ NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.

Original language	English
Pages (from-to)	1204-1207
Number of pages	4
Journal	Journal of the American Society for Mass Spectrometry
Volume	21
Issue number	7
DOIs	https://doi.org/10.1016/j.jasms.2010.02.028
Publication status	Published - 2010 Jul 1

All Science Journal Classification (ASJC) codes

Structural Biology
Spectroscopy

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title = "Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins",

abstract = "We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with {"}sweet spots{"}), when used in laser desorption/ionization mass spectrometry.",

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Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins. / Chiang, Cheng Kang; Chiang, Ni Chen; Lin, Zong Hong; Lan, Guo Yu; Lin, Yang Wei; Chang, Huan Tsung.

In: Journal of the American Society for Mass Spectrometry, Vol. 21, No. 7, 01.07.2010, p. 1204-1207.

Research output: Contribution to journal › Article › peer-review

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AU - Chang, Huan Tsung

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