Abstract
We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.
| Original language | English |
|---|---|
| Pages (from-to) | 1204-1207 |
| Number of pages | 4 |
| Journal | Journal of the American Society for Mass Spectrometry |
| Volume | 21 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2010 Jul 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Structural Biology
- Spectroscopy
Cite this
}
Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins. / Chiang, Cheng Kang; Chiang, Ni Chen; Lin, Zong Hong; Lan, Guo Yu; Lin, Yang Wei; Chang, Huan Tsung.
In: Journal of the American Society for Mass Spectrometry, Vol. 21, No. 7, 01.07.2010, p. 1204-1207.Research output: Contribution to journal › Article
TY - JOUR
T1 - Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins
AU - Chiang, Cheng Kang
AU - Chiang, Ni Chen
AU - Lin, Zong Hong
AU - Lan, Guo Yu
AU - Lin, Yang Wei
AU - Chang, Huan Tsung
PY - 2010/7/1
Y1 - 2010/7/1
N2 - We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.
AB - We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.
UR - http://www.scopus.com/inward/record.url?scp=77953913300&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953913300&partnerID=8YFLogxK
U2 - 10.1016/j.jasms.2010.02.028
DO - 10.1016/j.jasms.2010.02.028
M3 - Article
C2 - 20430643
AN - SCOPUS:77953913300
VL - 21
SP - 1204
EP - 1207
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
SN - 1044-0305
IS - 7
ER -