Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins

Cheng Kang Chiang, Ni Chen Chiang, Zong Hong Lin, Guo Yu Lan, Yang Wei Lin, Huan Tsung Chang

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We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.

Original languageEnglish
Pages (from-to)1204-1207
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Issue number7
Publication statusPublished - 2010 Jul 1


All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Spectroscopy

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