Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins

Cheng Kang Chiang, Ni Chen Chiang, Zong Hong Lin, Guo Yu Lan, Yang Wei Lin, Huan Tsung Chang

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.

Original languageEnglish
Pages (from-to)1204-1207
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Volume21
Issue number7
DOIs
Publication statusPublished - 2010 Jul 1

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Nanostructures
Nanostructured materials
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Peptides
Nanoparticles
Proteins
Gold
Glutathione
Limit of Detection
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Spectroscopy

Cite this

Chiang, Cheng Kang ; Chiang, Ni Chen ; Lin, Zong Hong ; Lan, Guo Yu ; Lin, Yang Wei ; Chang, Huan Tsung. / Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins. In: Journal of the American Society for Mass Spectrometry. 2010 ; Vol. 21, No. 7. pp. 1204-1207.
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Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and Proteins. / Chiang, Cheng Kang; Chiang, Ni Chen; Lin, Zong Hong; Lan, Guo Yu; Lin, Yang Wei; Chang, Huan Tsung.

In: Journal of the American Society for Mass Spectrometry, Vol. 21, No. 7, 01.07.2010, p. 1204-1207.

Research output: Contribution to journalArticle

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