Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in cervical cancer

Tsung Hsien Su, Jan Gowth Chang, Liuh I. Perng, Chih Peng Chang, Hsiao Jui Wei, Miau-Yaun Wang, Chang Hai Tsai

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective. PTEN/MMAC1, a candidate tumor suppressor gene located at chromosome 10q23.3, was recently identified and found to be homozygously deleted or mutated in several different types of human tumors. The aim of this study is to determine whether PTEN/MMAC1 is a target for 10q loss of heterozygosity in cervical cancer. Method. We examined 50 primary cervical carcinoma specimens using a PCR-based assay followed by SSCP and direct sequencing. The genomic DNA was also confirmed by Southern blot analysis. Results. All specimens except one, which has a 7-base deletion, showed a negative result. Among them, 30 randomly selected cases and their paired noncancerous tissue were further screened using nested RT-PCR. Six of 30 cervical cancerous tissues had aberrant transcripts. However, 4 of the matched noncancerous tissues also had aberrant transcripts. Southern blot analysis of the entire genomic DNA did not reveal any evidence of gene alteration. Conclusions. Sequence abnormalities in the PTEN/MMAC1 gene were only detected in 1 of 50 cervical cancers analyzed indicating that aberrant PTEN/MMAC1 function is an uncommon event in the development of cervix cancers. However, similar to studies with the TSG101 gene, screening for aberrant transcripts of PTEN/MMAC1 with nested RT-PCR may detect transcripts, which, although they vary from the normal size, may not be related to oncogenesis as they are also frequently found in normal tissues of the same patient.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalGynecologic Oncology
Volume76
Issue number2
DOIs
Publication statusPublished - 2000 Jan 1

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Tumor Suppressor Genes
Uterine Cervical Neoplasms
Southern Blotting
Polymerase Chain Reaction
Mutation
Genes
Single-Stranded Conformational Polymorphism
Choristoma
Loss of Heterozygosity
DNA
Carcinogenesis
Chromosomes
Carcinoma
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Obstetrics and Gynaecology

Cite this

Su, T. H., Chang, J. G., Perng, L. I., Chang, C. P., Wei, H. J., Wang, M-Y., & Tsai, C. H. (2000). Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in cervical cancer. Gynecologic Oncology, 76(2), 193-199. https://doi.org/10.1006/gyno.1999.5659
Su, Tsung Hsien ; Chang, Jan Gowth ; Perng, Liuh I. ; Chang, Chih Peng ; Wei, Hsiao Jui ; Wang, Miau-Yaun ; Tsai, Chang Hai. / Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in cervical cancer. In: Gynecologic Oncology. 2000 ; Vol. 76, No. 2. pp. 193-199.
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abstract = "Objective. PTEN/MMAC1, a candidate tumor suppressor gene located at chromosome 10q23.3, was recently identified and found to be homozygously deleted or mutated in several different types of human tumors. The aim of this study is to determine whether PTEN/MMAC1 is a target for 10q loss of heterozygosity in cervical cancer. Method. We examined 50 primary cervical carcinoma specimens using a PCR-based assay followed by SSCP and direct sequencing. The genomic DNA was also confirmed by Southern blot analysis. Results. All specimens except one, which has a 7-base deletion, showed a negative result. Among them, 30 randomly selected cases and their paired noncancerous tissue were further screened using nested RT-PCR. Six of 30 cervical cancerous tissues had aberrant transcripts. However, 4 of the matched noncancerous tissues also had aberrant transcripts. Southern blot analysis of the entire genomic DNA did not reveal any evidence of gene alteration. Conclusions. Sequence abnormalities in the PTEN/MMAC1 gene were only detected in 1 of 50 cervical cancers analyzed indicating that aberrant PTEN/MMAC1 function is an uncommon event in the development of cervix cancers. However, similar to studies with the TSG101 gene, screening for aberrant transcripts of PTEN/MMAC1 with nested RT-PCR may detect transcripts, which, although they vary from the normal size, may not be related to oncogenesis as they are also frequently found in normal tissues of the same patient.",
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Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in cervical cancer. / Su, Tsung Hsien; Chang, Jan Gowth; Perng, Liuh I.; Chang, Chih Peng; Wei, Hsiao Jui; Wang, Miau-Yaun; Tsai, Chang Hai.

In: Gynecologic Oncology, Vol. 76, No. 2, 01.01.2000, p. 193-199.

Research output: Contribution to journalArticle

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N2 - Objective. PTEN/MMAC1, a candidate tumor suppressor gene located at chromosome 10q23.3, was recently identified and found to be homozygously deleted or mutated in several different types of human tumors. The aim of this study is to determine whether PTEN/MMAC1 is a target for 10q loss of heterozygosity in cervical cancer. Method. We examined 50 primary cervical carcinoma specimens using a PCR-based assay followed by SSCP and direct sequencing. The genomic DNA was also confirmed by Southern blot analysis. Results. All specimens except one, which has a 7-base deletion, showed a negative result. Among them, 30 randomly selected cases and their paired noncancerous tissue were further screened using nested RT-PCR. Six of 30 cervical cancerous tissues had aberrant transcripts. However, 4 of the matched noncancerous tissues also had aberrant transcripts. Southern blot analysis of the entire genomic DNA did not reveal any evidence of gene alteration. Conclusions. Sequence abnormalities in the PTEN/MMAC1 gene were only detected in 1 of 50 cervical cancers analyzed indicating that aberrant PTEN/MMAC1 function is an uncommon event in the development of cervix cancers. However, similar to studies with the TSG101 gene, screening for aberrant transcripts of PTEN/MMAC1 with nested RT-PCR may detect transcripts, which, although they vary from the normal size, may not be related to oncogenesis as they are also frequently found in normal tissues of the same patient.

AB - Objective. PTEN/MMAC1, a candidate tumor suppressor gene located at chromosome 10q23.3, was recently identified and found to be homozygously deleted or mutated in several different types of human tumors. The aim of this study is to determine whether PTEN/MMAC1 is a target for 10q loss of heterozygosity in cervical cancer. Method. We examined 50 primary cervical carcinoma specimens using a PCR-based assay followed by SSCP and direct sequencing. The genomic DNA was also confirmed by Southern blot analysis. Results. All specimens except one, which has a 7-base deletion, showed a negative result. Among them, 30 randomly selected cases and their paired noncancerous tissue were further screened using nested RT-PCR. Six of 30 cervical cancerous tissues had aberrant transcripts. However, 4 of the matched noncancerous tissues also had aberrant transcripts. Southern blot analysis of the entire genomic DNA did not reveal any evidence of gene alteration. Conclusions. Sequence abnormalities in the PTEN/MMAC1 gene were only detected in 1 of 50 cervical cancers analyzed indicating that aberrant PTEN/MMAC1 function is an uncommon event in the development of cervix cancers. However, similar to studies with the TSG101 gene, screening for aberrant transcripts of PTEN/MMAC1 with nested RT-PCR may detect transcripts, which, although they vary from the normal size, may not be related to oncogenesis as they are also frequently found in normal tissues of the same patient.

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