Multiple molecular mechanisms cause reproductive isolation between three yeast species

Jui Yu Chou, Yin Shan Hung, Kuan Huei Lin, Hsin Yi Lee, Jun Yi Leu

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Nuclear-mitochondrial conflict (cytonuclear incompatibility) is a specific form of Dobzhansky-Muller incompatibility previously shown to cause reproductive isolation in two yeast species. Here, we identified two new incompatible genes, MRS1 and AIM22, through a systematic study of F2 hybrid sterility caused by cytonuclear incompatibility in three closely related Saccharomyces species (S. cerevisiae, S. paradoxus, and S. bayanus). Mrs1 is a nuclear gene product required for splicing specific introns in the mitochondrial COX1, and Aim22 is a ligase encoded in the nucleus that is required for mitochondrial protein lipoylation. By comparing different species, our result suggests that the functional changes in MRS1 are a result of coevolution with changes in the COX1 introns. Further molecular analyses demonstrate that three nonsynonymous mutations are responsible for the functional differences of Mrs1 between these species. Functional complementation assays to determine when these incompatible genes altered their functions show a strong correlation between the sequence-based phylogeny and the evolution of cytonuclear incompatibility. Our results suggest that nuclearmitochondrial incompatibility may represent a general mechanism of reproductive isolation during yeast evolution.

Original languageEnglish
JournalPLoS Biology
Volume8
Issue number7
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

Reproductive Isolation
reproductive isolation
Yeast
Genes
Yeasts
yeasts
Introns
introns
Lipoylation
Saccharomyces
genes
Mitochondrial Proteins
Phylogeny
Ligases
coevolution
ligases
Infertility
Saccharomyces cerevisiae
Assays
mutation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Chou, Jui Yu ; Hung, Yin Shan ; Lin, Kuan Huei ; Lee, Hsin Yi ; Leu, Jun Yi. / Multiple molecular mechanisms cause reproductive isolation between three yeast species. In: PLoS Biology. 2010 ; Vol. 8, No. 7.
@article{0fe1c5a932a545b991f7ffa8b64fb99b,
title = "Multiple molecular mechanisms cause reproductive isolation between three yeast species",
abstract = "Nuclear-mitochondrial conflict (cytonuclear incompatibility) is a specific form of Dobzhansky-Muller incompatibility previously shown to cause reproductive isolation in two yeast species. Here, we identified two new incompatible genes, MRS1 and AIM22, through a systematic study of F2 hybrid sterility caused by cytonuclear incompatibility in three closely related Saccharomyces species (S. cerevisiae, S. paradoxus, and S. bayanus). Mrs1 is a nuclear gene product required for splicing specific introns in the mitochondrial COX1, and Aim22 is a ligase encoded in the nucleus that is required for mitochondrial protein lipoylation. By comparing different species, our result suggests that the functional changes in MRS1 are a result of coevolution with changes in the COX1 introns. Further molecular analyses demonstrate that three nonsynonymous mutations are responsible for the functional differences of Mrs1 between these species. Functional complementation assays to determine when these incompatible genes altered their functions show a strong correlation between the sequence-based phylogeny and the evolution of cytonuclear incompatibility. Our results suggest that nuclearmitochondrial incompatibility may represent a general mechanism of reproductive isolation during yeast evolution.",
author = "Chou, {Jui Yu} and Hung, {Yin Shan} and Lin, {Kuan Huei} and Lee, {Hsin Yi} and Leu, {Jun Yi}",
year = "2010",
month = "7",
day = "1",
doi = "10.1371/journal.pbio.1000432",
language = "English",
volume = "8",
journal = "PLoS Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "7",

}

Multiple molecular mechanisms cause reproductive isolation between three yeast species. / Chou, Jui Yu; Hung, Yin Shan; Lin, Kuan Huei; Lee, Hsin Yi; Leu, Jun Yi.

In: PLoS Biology, Vol. 8, No. 7, 01.07.2010.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multiple molecular mechanisms cause reproductive isolation between three yeast species

AU - Chou, Jui Yu

AU - Hung, Yin Shan

AU - Lin, Kuan Huei

AU - Lee, Hsin Yi

AU - Leu, Jun Yi

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Nuclear-mitochondrial conflict (cytonuclear incompatibility) is a specific form of Dobzhansky-Muller incompatibility previously shown to cause reproductive isolation in two yeast species. Here, we identified two new incompatible genes, MRS1 and AIM22, through a systematic study of F2 hybrid sterility caused by cytonuclear incompatibility in three closely related Saccharomyces species (S. cerevisiae, S. paradoxus, and S. bayanus). Mrs1 is a nuclear gene product required for splicing specific introns in the mitochondrial COX1, and Aim22 is a ligase encoded in the nucleus that is required for mitochondrial protein lipoylation. By comparing different species, our result suggests that the functional changes in MRS1 are a result of coevolution with changes in the COX1 introns. Further molecular analyses demonstrate that three nonsynonymous mutations are responsible for the functional differences of Mrs1 between these species. Functional complementation assays to determine when these incompatible genes altered their functions show a strong correlation between the sequence-based phylogeny and the evolution of cytonuclear incompatibility. Our results suggest that nuclearmitochondrial incompatibility may represent a general mechanism of reproductive isolation during yeast evolution.

AB - Nuclear-mitochondrial conflict (cytonuclear incompatibility) is a specific form of Dobzhansky-Muller incompatibility previously shown to cause reproductive isolation in two yeast species. Here, we identified two new incompatible genes, MRS1 and AIM22, through a systematic study of F2 hybrid sterility caused by cytonuclear incompatibility in three closely related Saccharomyces species (S. cerevisiae, S. paradoxus, and S. bayanus). Mrs1 is a nuclear gene product required for splicing specific introns in the mitochondrial COX1, and Aim22 is a ligase encoded in the nucleus that is required for mitochondrial protein lipoylation. By comparing different species, our result suggests that the functional changes in MRS1 are a result of coevolution with changes in the COX1 introns. Further molecular analyses demonstrate that three nonsynonymous mutations are responsible for the functional differences of Mrs1 between these species. Functional complementation assays to determine when these incompatible genes altered their functions show a strong correlation between the sequence-based phylogeny and the evolution of cytonuclear incompatibility. Our results suggest that nuclearmitochondrial incompatibility may represent a general mechanism of reproductive isolation during yeast evolution.

UR - http://www.scopus.com/inward/record.url?scp=77955041686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955041686&partnerID=8YFLogxK

U2 - 10.1371/journal.pbio.1000432

DO - 10.1371/journal.pbio.1000432

M3 - Article

C2 - 20652018

AN - SCOPUS:77955041686

VL - 8

JO - PLoS Biology

JF - PLoS Biology

SN - 1544-9173

IS - 7

ER -