Molecular characterization of the early response of orchid phalaenopsis amabilis to erwinia chrysanthemi infection

Shih Feng Fu, Hao Jen Huang

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Erwinia chrysanthemi is a devastating bacterial pathogen in orchid horticulture and causes soft-rot diseases by secretion of cell wall-degrading enzymes. However, the pathogenesis and resistance response to the pathogen in the orchid remain to be elucidated. In this chapter, the molecular events of the early host–pathogen interaction between Phalaenopsis orchids and E. chrysanthemi are described on the basis of gene expression profiles. At the early stage of pathogenesis, the defense mechanism, such as an alteration in reactive oxygen species level, was elicited upon infection. For the analysis of the signal transduction pathway triggered by E. chrysanthemi infection, a PCR-based strategy using degenerate primers was performed to identify the genes encoding protein kinases and phosphatases. The genes coding for Phalaenopsis amabilis calcium-dependent proteinkinase 1 (PaCDPK1) and tyrosine phosphatase (PaPTP1) were isolated from the orchid, and their expression in response to pathogen infection and wounding were characterized. To gain more insight into the mechanisms underlying the compatible interactions, genome-wide transcriptional regulation was investigated by a suppression subtractive hybridization strategy. A total of 170 clones were sequenced from the whole cDNA library to generate 65 nonredundant pathogen-responsive transcripts. Among the genes with assigned function, 37 percent were associated with metabolism, 30 percent with environmental interactions, 13 percent with energy, and 20 percent with others. One of the pathogen-responsive transcripts encoding trans-2-enoyl-CoA reductase was the most abundant representative of plant genes, comprising 15 percent of the total clones. A putative model of the early molecular events in the orchid in response to E. chrysanthemi infection was proposed. The chapter provides valuable information towards the understanding the orchid—E. chrysanthemi interaction at the molecular level.

Original languageEnglish
Title of host publicationOrchid Biotechnology II
PublisherWorld Scientific Publishing Co.
Pages283-308
Number of pages26
ISBN (Electronic)9789814327930
ISBN (Print)9814327921, 9789814327923
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

Pectobacterium chrysanthemi
Orchidaceae
Erwinia chrysanthemi
Phalaenopsis
Pathogens
Genes
pathogens
Infection
infection
Clone Cells
Fatty Acid Desaturases
Plant Genes
genes
pathogenesis
Molecular Models
Phosphoprotein Phosphatases
clones
Signal transduction
Gene Library
Transcriptome

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fu, Shih Feng ; Huang, Hao Jen. / Molecular characterization of the early response of orchid phalaenopsis amabilis to erwinia chrysanthemi infection. Orchid Biotechnology II. World Scientific Publishing Co., 2011. pp. 283-308
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Molecular characterization of the early response of orchid phalaenopsis amabilis to erwinia chrysanthemi infection. / Fu, Shih Feng; Huang, Hao Jen.

Orchid Biotechnology II. World Scientific Publishing Co., 2011. p. 283-308.

Research output: Chapter in Book/Report/Conference proceedingChapter

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