Molecular characterization and gene expression pattern of two putative molt-inhibiting hormones from Litopenaeus vannamei

Hsiang Yin Chen, R. Douglas Watson, Jiann Chu Chen, Hui Fen Liu, Chi-Ying Lee

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Two cDNA sequences (Liv-MIH1 and Liv-MIH2) were cloned from the eyestalk ganglia of the white shrimp Litopenaeus vannamei. The conceptually translated peptide precursors consist of a mature peptide (77 residues for Liv-MIH1, 75 residues for Liv-MIH2), preceded by a 28-residue signal peptide. Both mature peptides share highest sequence identity with other known MIHs, and contain several conserved residues that have been proposed to be functionally critical for MIH activity. Analysis of genomic sequences reveals that both genes are organized in a 3 exon/2 intron manner, with the same sites of intron insertion. The transcripts of Liv-MIH1 and Liv-MIH2 were detected exclusively in the eyestalk, but not in other neural and non-neural tissues examined. Phylogenetic analysis indicates that Liv-MIH1 and Liv-MIH2 cluster with the type II peptides that are considered as penaeid MIH. In addition, a quantitative real-time polymerase chain reaction (PCR) assay was developed and validated for the quantification of gene expression of Liv-MIH1 and Liv-MIH2. Transcript levels for both genes remained constant through stages A - D1 (ranges of relative expression levels are 97.9 ± 2.9 to 104.5 ± 8.9% for Liv-MIH1, and 85.6 ± 6.7 to 104.7 ± 10.8% for Liv-MIH2), and declined afterwards, reaching a lowest level during stage D2D3 (40.6 ± 0.4% for Liv-MIH1, and 48.5 ± 3.2% for Liv-MIH2). These significant decreases in the transcript levels correspond to a significant increase in hemolymph ecdysteroid titers at stage D2D3. These results clearly indicate that Liv-MIH1 and Liv-MIH2 are type II peptides of the crustacean hyperglycemic hormone family and most likely function as MIHs in the white shrimp. They are discussed with regard to the presence of multiple MIHs and possible functional divergence of type II peptides in Penaeidae, as well as endocrine regulation of crustacean molting.

Original languageEnglish
Pages (from-to)72-81
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume151
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Litopenaeus vannamei
molting
hormones
peptides
Gene Expression
gene expression
Peptides
Introns
introns
shrimp
Crustacea
Penaeidae
Ecdysteroids
Molting
Hemolymph
ecdysteroids
Protein Sorting Signals
signal peptide
Ganglia
Genes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Endocrinology

Cite this

Chen, Hsiang Yin ; Douglas Watson, R. ; Chen, Jiann Chu ; Liu, Hui Fen ; Lee, Chi-Ying. / Molecular characterization and gene expression pattern of two putative molt-inhibiting hormones from Litopenaeus vannamei. In: General and Comparative Endocrinology. 2007 ; Vol. 151, No. 1. pp. 72-81.
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abstract = "Two cDNA sequences (Liv-MIH1 and Liv-MIH2) were cloned from the eyestalk ganglia of the white shrimp Litopenaeus vannamei. The conceptually translated peptide precursors consist of a mature peptide (77 residues for Liv-MIH1, 75 residues for Liv-MIH2), preceded by a 28-residue signal peptide. Both mature peptides share highest sequence identity with other known MIHs, and contain several conserved residues that have been proposed to be functionally critical for MIH activity. Analysis of genomic sequences reveals that both genes are organized in a 3 exon/2 intron manner, with the same sites of intron insertion. The transcripts of Liv-MIH1 and Liv-MIH2 were detected exclusively in the eyestalk, but not in other neural and non-neural tissues examined. Phylogenetic analysis indicates that Liv-MIH1 and Liv-MIH2 cluster with the type II peptides that are considered as penaeid MIH. In addition, a quantitative real-time polymerase chain reaction (PCR) assay was developed and validated for the quantification of gene expression of Liv-MIH1 and Liv-MIH2. Transcript levels for both genes remained constant through stages A - D1′ (ranges of relative expression levels are 97.9 ± 2.9 to 104.5 ± 8.9{\%} for Liv-MIH1, and 85.6 ± 6.7 to 104.7 ± 10.8{\%} for Liv-MIH2), and declined afterwards, reaching a lowest level during stage D2D3 (40.6 ± 0.4{\%} for Liv-MIH1, and 48.5 ± 3.2{\%} for Liv-MIH2). These significant decreases in the transcript levels correspond to a significant increase in hemolymph ecdysteroid titers at stage D2D3. These results clearly indicate that Liv-MIH1 and Liv-MIH2 are type II peptides of the crustacean hyperglycemic hormone family and most likely function as MIHs in the white shrimp. They are discussed with regard to the presence of multiple MIHs and possible functional divergence of type II peptides in Penaeidae, as well as endocrine regulation of crustacean molting.",
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Molecular characterization and gene expression pattern of two putative molt-inhibiting hormones from Litopenaeus vannamei. / Chen, Hsiang Yin; Douglas Watson, R.; Chen, Jiann Chu; Liu, Hui Fen; Lee, Chi-Ying.

In: General and Comparative Endocrinology, Vol. 151, No. 1, 01.01.2007, p. 72-81.

Research output: Contribution to journalArticle

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AU - Chen, Hsiang Yin

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