Abstract
In this study, a sensitive and selective fluorescence assay using the microwave-assisted synthesis of bovine serum albumin-stabilised gold nanoclusters (MW-BSA-AuNCs) was proposed for the determination of lead(ii) and melamine in aqueous solution. The fluorescence of the MW-BSA-AuNCs was quenched through a Pb2+-mediated interparticle aggregation mechanism, and thus the change in fluorescence at 660 nm was dependent on the Pb2+ ion concentration. In addition, the fluorescence of the MW-BSA-AuNCs was also quenched on the basis of the high-affinity metallophilic Hg2+-Au+ interaction, whereas the fluorescence of the MW-BSA-AuNCs was restored when melamine was added and coordinated with Hg2+. Under optimal conditions, the limits of detection for Pb2+ ions and melamine at a signal-to-noise ratio of 3 were 4.8 nM and 2.94 μM, respectively. Our present approach is simpler and more cost-effective than the existing techniques for the detection of Pb2+ ions in seawater and aqueous urine samples and of melamine in milk samples.
| Original language | English |
|---|---|
| Pages (from-to) | 79020-79027 |
| Number of pages | 8 |
| Journal | RSC Advances |
| Volume | 6 |
| Issue number | 82 |
| DOIs | |
| Publication status | Published - 2016 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)
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Microwave-assisted synthesis of BSA-stabilised gold nanoclusters for the sensitive and selective detection of lead(II) and melamine in aqueous solution. / Lee, Che Yu; Hsu, Nai Yueh; Wu, Mei Yao; Lin, Yang Wei.
In: RSC Advances, Vol. 6, No. 82, 01.01.2016, p. 79020-79027.Research output: Contribution to journal › Article
TY - JOUR
T1 - Microwave-assisted synthesis of BSA-stabilised gold nanoclusters for the sensitive and selective detection of lead(II) and melamine in aqueous solution
AU - Lee, Che Yu
AU - Hsu, Nai Yueh
AU - Wu, Mei Yao
AU - Lin, Yang Wei
PY - 2016/1/1
Y1 - 2016/1/1
N2 - In this study, a sensitive and selective fluorescence assay using the microwave-assisted synthesis of bovine serum albumin-stabilised gold nanoclusters (MW-BSA-AuNCs) was proposed for the determination of lead(ii) and melamine in aqueous solution. The fluorescence of the MW-BSA-AuNCs was quenched through a Pb2+-mediated interparticle aggregation mechanism, and thus the change in fluorescence at 660 nm was dependent on the Pb2+ ion concentration. In addition, the fluorescence of the MW-BSA-AuNCs was also quenched on the basis of the high-affinity metallophilic Hg2+-Au+ interaction, whereas the fluorescence of the MW-BSA-AuNCs was restored when melamine was added and coordinated with Hg2+. Under optimal conditions, the limits of detection for Pb2+ ions and melamine at a signal-to-noise ratio of 3 were 4.8 nM and 2.94 μM, respectively. Our present approach is simpler and more cost-effective than the existing techniques for the detection of Pb2+ ions in seawater and aqueous urine samples and of melamine in milk samples.
AB - In this study, a sensitive and selective fluorescence assay using the microwave-assisted synthesis of bovine serum albumin-stabilised gold nanoclusters (MW-BSA-AuNCs) was proposed for the determination of lead(ii) and melamine in aqueous solution. The fluorescence of the MW-BSA-AuNCs was quenched through a Pb2+-mediated interparticle aggregation mechanism, and thus the change in fluorescence at 660 nm was dependent on the Pb2+ ion concentration. In addition, the fluorescence of the MW-BSA-AuNCs was also quenched on the basis of the high-affinity metallophilic Hg2+-Au+ interaction, whereas the fluorescence of the MW-BSA-AuNCs was restored when melamine was added and coordinated with Hg2+. Under optimal conditions, the limits of detection for Pb2+ ions and melamine at a signal-to-noise ratio of 3 were 4.8 nM and 2.94 μM, respectively. Our present approach is simpler and more cost-effective than the existing techniques for the detection of Pb2+ ions in seawater and aqueous urine samples and of melamine in milk samples.
UR - http://www.scopus.com/inward/record.url?scp=84984698683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84984698683&partnerID=8YFLogxK
U2 - 10.1039/c6ra16043c
DO - 10.1039/c6ra16043c
M3 - Article
AN - SCOPUS:84984698683
VL - 6
SP - 79020
EP - 79027
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 82
ER -