Microwave-assisted synthesis of BSA-stabilised gold nanoclusters for the sensitive and selective detection of lead(II) and melamine in aqueous solution

Che Yu Lee, Nai Yueh Hsu, Mei Yao Wu, Yang Wei Lin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, a sensitive and selective fluorescence assay using the microwave-assisted synthesis of bovine serum albumin-stabilised gold nanoclusters (MW-BSA-AuNCs) was proposed for the determination of lead(ii) and melamine in aqueous solution. The fluorescence of the MW-BSA-AuNCs was quenched through a Pb2+-mediated interparticle aggregation mechanism, and thus the change in fluorescence at 660 nm was dependent on the Pb2+ ion concentration. In addition, the fluorescence of the MW-BSA-AuNCs was also quenched on the basis of the high-affinity metallophilic Hg2+-Au+ interaction, whereas the fluorescence of the MW-BSA-AuNCs was restored when melamine was added and coordinated with Hg2+. Under optimal conditions, the limits of detection for Pb2+ ions and melamine at a signal-to-noise ratio of 3 were 4.8 nM and 2.94 μM, respectively. Our present approach is simpler and more cost-effective than the existing techniques for the detection of Pb2+ ions in seawater and aqueous urine samples and of melamine in milk samples.

Original languageEnglish
Pages (from-to)79020-79027
Number of pages8
JournalRSC Advances
Volume6
Issue number82
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Melamine
Nanoclusters
Gold
Lead
Fluorescence
Microwaves
Ions
Bovine Serum Albumin
Seawater
Assays
Signal to noise ratio
Agglomeration
melamine
Costs

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

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title = "Microwave-assisted synthesis of BSA-stabilised gold nanoclusters for the sensitive and selective detection of lead(II) and melamine in aqueous solution",
abstract = "In this study, a sensitive and selective fluorescence assay using the microwave-assisted synthesis of bovine serum albumin-stabilised gold nanoclusters (MW-BSA-AuNCs) was proposed for the determination of lead(ii) and melamine in aqueous solution. The fluorescence of the MW-BSA-AuNCs was quenched through a Pb2+-mediated interparticle aggregation mechanism, and thus the change in fluorescence at 660 nm was dependent on the Pb2+ ion concentration. In addition, the fluorescence of the MW-BSA-AuNCs was also quenched on the basis of the high-affinity metallophilic Hg2+-Au+ interaction, whereas the fluorescence of the MW-BSA-AuNCs was restored when melamine was added and coordinated with Hg2+. Under optimal conditions, the limits of detection for Pb2+ ions and melamine at a signal-to-noise ratio of 3 were 4.8 nM and 2.94 μM, respectively. Our present approach is simpler and more cost-effective than the existing techniques for the detection of Pb2+ ions in seawater and aqueous urine samples and of melamine in milk samples.",
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Microwave-assisted synthesis of BSA-stabilised gold nanoclusters for the sensitive and selective detection of lead(II) and melamine in aqueous solution. / Lee, Che Yu; Hsu, Nai Yueh; Wu, Mei Yao; Lin, Yang Wei.

In: RSC Advances, Vol. 6, No. 82, 01.01.2016, p. 79020-79027.

Research output: Contribution to journalArticle

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