Microwave-assisted synthesis of BSA-stabilised gold nanoclusters for the sensitive and selective detection of lead(II) and melamine in aqueous solution

Che Yu Lee, Nai Yueh Hsu, Mei Yao Wu, Yang Wei Lin

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7 Citations (Scopus)


In this study, a sensitive and selective fluorescence assay using the microwave-assisted synthesis of bovine serum albumin-stabilised gold nanoclusters (MW-BSA-AuNCs) was proposed for the determination of lead(ii) and melamine in aqueous solution. The fluorescence of the MW-BSA-AuNCs was quenched through a Pb2+-mediated interparticle aggregation mechanism, and thus the change in fluorescence at 660 nm was dependent on the Pb2+ ion concentration. In addition, the fluorescence of the MW-BSA-AuNCs was also quenched on the basis of the high-affinity metallophilic Hg2+-Au+ interaction, whereas the fluorescence of the MW-BSA-AuNCs was restored when melamine was added and coordinated with Hg2+. Under optimal conditions, the limits of detection for Pb2+ ions and melamine at a signal-to-noise ratio of 3 were 4.8 nM and 2.94 μM, respectively. Our present approach is simpler and more cost-effective than the existing techniques for the detection of Pb2+ ions in seawater and aqueous urine samples and of melamine in milk samples.

Original languageEnglish
Pages (from-to)79020-79027
Number of pages8
JournalRSC Advances
Issue number82
Publication statusPublished - 2016 Jan 1


All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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