Microbial community structure during oxygen-stimulated bioremediation in phenol-contaminated groundwater

Chi Wen Lin, Chi Yung Lai, Li Hsuan Chen, Wan Fu Chiang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This research explored the changes in genetic diversity and spatial distribution of microbial communities in association with the changes in phenol concentration during a bioremediation process. Results using the traditional plate count method indicated an increase of average bacteria densities in groundwater from 104 to 107 CFU ml-1 initially to 107 to 109 CFU ml-1 after remediation. The diversity and stability of phenol-degrading bacterial communities were investigated by using single-strand-conformation polymorphism (SSCP) genetic profile analysis of 16S rDNA fragments amplified from groundwater samples. The molecular data showed a high degree of genetic similarity between communities from certain monitoring wells during the early phases of remediation, probably due to similar initial physical conditions among wells. Molecular signatures of several cultivated phenol-degrading bacterial strains could be seen in most groundwater profiles throughout the study period, suggesting that these strains were indigenous to the study site. It was also observed that the species diversity of these microbial communities increased as the phenol levels in the groundwater decreased during the 9-month study period, and recovered to the pre-treatment levels after the remediation program was completed.

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalJournal of Hazardous Materials
Volume140
Issue number1-2
DOIs
Publication statusPublished - 2007 Feb 9

Fingerprint

Environmental Biodegradation
Bioremediation
Groundwater
Phenol
bioremediation
Phenols
phenol
microbial community
community structure
Remediation
Oxygen
oxygen
remediation
groundwater
well
Biodiversity
Ribosomal DNA
Polymorphism
Spatial distribution
Conformations

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

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abstract = "This research explored the changes in genetic diversity and spatial distribution of microbial communities in association with the changes in phenol concentration during a bioremediation process. Results using the traditional plate count method indicated an increase of average bacteria densities in groundwater from 104 to 107 CFU ml-1 initially to 107 to 109 CFU ml-1 after remediation. The diversity and stability of phenol-degrading bacterial communities were investigated by using single-strand-conformation polymorphism (SSCP) genetic profile analysis of 16S rDNA fragments amplified from groundwater samples. The molecular data showed a high degree of genetic similarity between communities from certain monitoring wells during the early phases of remediation, probably due to similar initial physical conditions among wells. Molecular signatures of several cultivated phenol-degrading bacterial strains could be seen in most groundwater profiles throughout the study period, suggesting that these strains were indigenous to the study site. It was also observed that the species diversity of these microbial communities increased as the phenol levels in the groundwater decreased during the 9-month study period, and recovered to the pre-treatment levels after the remediation program was completed.",
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Microbial community structure during oxygen-stimulated bioremediation in phenol-contaminated groundwater. / Lin, Chi Wen; Lai, Chi Yung; Chen, Li Hsuan; Chiang, Wan Fu.

In: Journal of Hazardous Materials, Vol. 140, No. 1-2, 09.02.2007, p. 221-229.

Research output: Contribution to journalArticle

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