Microbial community analysis in the termite gut and fungus comb of Odontotermes formosanus: The implication of Bacillus as mutualists

Gincy Marina Mathew, Yu Ming Ju, Chi Yung Lai, Dony Chacko Mathew, Chieh Chen Huang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The microbial communities harbored in the gut and fungus comb of the fungus-growing termite Odontotermes formosanus were analyzed by both culture-dependent and culture-independent methods to better understand the community structure of their microflora. The microorganisms detected by denaturing gradient gel electrophoresis (DGGE), clonal selection, and culture-dependent methods were hypothesized to contribute to cellulose-hemicellulose hydrolysis, gut fermentation, nutrient production, the breakdown of the fungus comb and the initiation of the growth of the symbiotic fungus Termitomyces. The predominant bacterial cultivars isolated by the cultural approach belonged to the genus Bacillus (Phylum Firmicutes). Apart from their function in lignocellulosic degradation, the Bacillus isolates suppressed the growth of the microfungus Trichoderma harzianum (genus Hypocrea), which grew voraciously on the fungus comb in the absence of termites but grew in harmony with the symbiotic fungus Termitomyces. The in vitro studies suggested that the Bacillus sp. may function as mutualists in the termite-gut-fungus-comb microbial ecosystem.

Original languageEnglish
Pages (from-to)504-517
Number of pages14
JournalFEMS Microbiology Ecology
Volume79
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

Isoptera
Comb and Wattles
termite
Bacillus
microbial community
Fungi
fungus
Termitomyces
Hypocrea
Denaturing Gradient Gel Electrophoresis
Trichoderma
Growth
analysis
Cellulose
Fermentation
fermentation
Ecosystem
cellulose
cultivar
hydrolysis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Mathew, Gincy Marina ; Ju, Yu Ming ; Lai, Chi Yung ; Mathew, Dony Chacko ; Huang, Chieh Chen. / Microbial community analysis in the termite gut and fungus comb of Odontotermes formosanus : The implication of Bacillus as mutualists. In: FEMS Microbiology Ecology. 2012 ; Vol. 79, No. 2. pp. 504-517.
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Microbial community analysis in the termite gut and fungus comb of Odontotermes formosanus : The implication of Bacillus as mutualists. / Mathew, Gincy Marina; Ju, Yu Ming; Lai, Chi Yung; Mathew, Dony Chacko; Huang, Chieh Chen.

In: FEMS Microbiology Ecology, Vol. 79, No. 2, 01.02.2012, p. 504-517.

Research output: Contribution to journalArticle

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