Abstract
The distribution of nitric oxide synthase (NOS) in the eyestalk tissues of the crayfish, Procambarus clarkii, was studied using NADPH-diaphorase (NADPH-d) histochemistry and an NOS biochemical assay. In the retina, NADPH-d activity was detected in the rhabdom of the ommatidium and axons of retinular cells that terminate in the lamina ganglionaris. In the eyestalk ganglia, NADPH-d was localized to the fibers in the 1st chiasma that connect the lamina ganglionaris and medulla externa, and 2 prominent fiber tracts that appear to originate from the medulla terminalis, project distally, and terminate in the synaptic layers of the medulla interna. In addition, NIADPH-d-positive somata were found in the periphery of the medulla externa. Specific staining of NADPH-d activity was eliminated by DPIP, an inhibitor of NOS-associated NADPH-d. The distribution pattern of NADPH-d activity described above is much broader than that of NOS-immunoreactivity reported in a previous study (Lee et al. 2000). Biochemical analysis revealed that the regions (the medulla interna and medulla terminalis) where only NADPH-d activity (but not NOS-immunoreactivity) was detected also contained significant levels of NOS activity, suggesting that the observed NADPH-d activity is associated with NOS. The combined results indicate that, in contrast to a highly restricted pattern suggested by immunohistochemical data, NOS is widely distributed in the crayfish eyestalk and implicates NO in the processing of visual information.
| Original language | English |
|---|---|
| Pages (from-to) | 244-250 |
| Number of pages | 7 |
| Journal | Zoological Studies |
| Volume | 41 |
| Issue number | 3 |
| Publication status | Published - 2002 Jul 1 |
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All Science Journal Classification (ASJC) codes
- Animal Science and Zoology
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Localization of NADPH-diaphorase and nitric oxide synthase activity in the eyestalk of the crayfish, Procambarus clarkii. / Zou, Hong Shin; Chang, Yi Zhen; Chen, Shyh Chi; Yau, Shiou Mei; Shen, Ya Lun; Lee, Chi Ying.
In: Zoological Studies, Vol. 41, No. 3, 01.07.2002, p. 244-250.Research output: Contribution to journal › Article
TY - JOUR
T1 - Localization of NADPH-diaphorase and nitric oxide synthase activity in the eyestalk of the crayfish, Procambarus clarkii
AU - Zou, Hong Shin
AU - Chang, Yi Zhen
AU - Chen, Shyh Chi
AU - Yau, Shiou Mei
AU - Shen, Ya Lun
AU - Lee, Chi Ying
PY - 2002/7/1
Y1 - 2002/7/1
N2 - The distribution of nitric oxide synthase (NOS) in the eyestalk tissues of the crayfish, Procambarus clarkii, was studied using NADPH-diaphorase (NADPH-d) histochemistry and an NOS biochemical assay. In the retina, NADPH-d activity was detected in the rhabdom of the ommatidium and axons of retinular cells that terminate in the lamina ganglionaris. In the eyestalk ganglia, NADPH-d was localized to the fibers in the 1st chiasma that connect the lamina ganglionaris and medulla externa, and 2 prominent fiber tracts that appear to originate from the medulla terminalis, project distally, and terminate in the synaptic layers of the medulla interna. In addition, NIADPH-d-positive somata were found in the periphery of the medulla externa. Specific staining of NADPH-d activity was eliminated by DPIP, an inhibitor of NOS-associated NADPH-d. The distribution pattern of NADPH-d activity described above is much broader than that of NOS-immunoreactivity reported in a previous study (Lee et al. 2000). Biochemical analysis revealed that the regions (the medulla interna and medulla terminalis) where only NADPH-d activity (but not NOS-immunoreactivity) was detected also contained significant levels of NOS activity, suggesting that the observed NADPH-d activity is associated with NOS. The combined results indicate that, in contrast to a highly restricted pattern suggested by immunohistochemical data, NOS is widely distributed in the crayfish eyestalk and implicates NO in the processing of visual information.
AB - The distribution of nitric oxide synthase (NOS) in the eyestalk tissues of the crayfish, Procambarus clarkii, was studied using NADPH-diaphorase (NADPH-d) histochemistry and an NOS biochemical assay. In the retina, NADPH-d activity was detected in the rhabdom of the ommatidium and axons of retinular cells that terminate in the lamina ganglionaris. In the eyestalk ganglia, NADPH-d was localized to the fibers in the 1st chiasma that connect the lamina ganglionaris and medulla externa, and 2 prominent fiber tracts that appear to originate from the medulla terminalis, project distally, and terminate in the synaptic layers of the medulla interna. In addition, NIADPH-d-positive somata were found in the periphery of the medulla externa. Specific staining of NADPH-d activity was eliminated by DPIP, an inhibitor of NOS-associated NADPH-d. The distribution pattern of NADPH-d activity described above is much broader than that of NOS-immunoreactivity reported in a previous study (Lee et al. 2000). Biochemical analysis revealed that the regions (the medulla interna and medulla terminalis) where only NADPH-d activity (but not NOS-immunoreactivity) was detected also contained significant levels of NOS activity, suggesting that the observed NADPH-d activity is associated with NOS. The combined results indicate that, in contrast to a highly restricted pattern suggested by immunohistochemical data, NOS is widely distributed in the crayfish eyestalk and implicates NO in the processing of visual information.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036394380&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0036394380
VL - 41
SP - 244
EP - 250
JO - Zoological Studies
JF - Zoological Studies
SN - 1021-5506
IS - 3
ER -